真核生物核糖体上tRNA的结合位点。

The binding sites for tRNA on eukaryotic ribosomes.

作者信息

Leader D P, Machray G C

出版信息

Nucleic Acids Res. 1975 Jul;2(7):1177-88. doi: 10.1093/nar/2.7.1177.

DOI:10.1093/nar/2.7.1177

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343504/

Abstract

We have studied the non-enzymic binding of phe-tRNA to ribosomes from rat liver using deacylated tRNA to inhibit binding to the P-site and puromycin (5 x 10-minus3M) to inhibit binding to the A-site. We conclude that at a low concentration of magnesium ions (10mM) phe-tRNA is bound only at the A-site of 80S irbosomes, whereas at a high concentration of magnesium ions (40mM) phe-tRNA is also bound at the P-site. Studies with edeine indicate that, during non-enzymic binding of phe-tRNA, eukaryotic ribosomes (in contrast to prokarotic ribosomes) have the A-site of the 60S subunit and the initiation site of the 40S subunit juxtaposed. This may account for the differences observed, in formation of diphenylalanyl-tRNA and phenylalanyl-puromycin, between phe-tRNA bound non-enzymically to the P-sites of eukaryotic and prokaryotic ribosomes.

摘要

我们使用脱酰基tRNA抑制与P位点的结合以及嘌呤霉素（5×10⁻³M）抑制与A位点的结合，研究了苯丙氨酰-tRNA与大鼠肝脏核糖体的非酶促结合。我们得出结论，在低浓度镁离子（10mM）下，苯丙氨酰-tRNA仅结合于80S核糖体的A位点，而在高浓度镁离子（40mM）下，苯丙氨酰-tRNA也结合于P位点。用伊短菌素进行的研究表明，在苯丙氨酰-tRNA的非酶促结合过程中，真核核糖体（与原核核糖体不同）的60S亚基的A位点和40S亚基的起始位点并列。这可能解释了在非酶促结合于真核和原核核糖体P位点的苯丙氨酰-tRNA之间，在二苯丙氨酰-tRNA和苯丙氨酰-嘌呤霉素形成过程中观察到的差异。

相似文献

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The binding sites for tRNA on eukaryotic ribosomes.真核生物核糖体上tRNA的结合位点。

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2

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本文引用的文献

1

THE SYNTHESIS OF PROTEINS UPON RIBOSOMES.核糖体上蛋白质的合成。

Bull Soc Chim Biol (Paris). 1964;46:1399-425.

2

RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES.RNA密码子与蛋白质合成。三核苷酸对可溶性核糖核酸（sRNA）与核糖体结合的影响。

Science. 1964 Sep 25;145(3639):1399-407. doi: 10.1126/science.145.3639.1399.

3

Translocation in protein synthesis: a hybrid structure model.蛋白质合成中的易位：一种杂交结构模型。

Nature. 1968 May 18;218(5142):675-7. doi: 10.1038/218675a0.

4

Inhibition of peptide initiation on reticulocyte ribosomes by edeine.伊短菌素对网织红细胞核糖体上肽链起始的抑制作用。

Eur J Biochem. 1971 Jul 15;21(1):31-41. doi: 10.1111/j.1432-1033.1971.tb01436.x.

5

Effect of edeine on aminoacyl-tRNA binding to ribosomes and its relationship to ribosomal binding sites.伊短菌素对氨酰tRNA与核糖体结合的影响及其与核糖体结合位点的关系。

Biochim Biophys Acta. 1970 Dec 14;224(2):477-86. doi: 10.1016/0005-2787(70)90580-0.

6

Initiation of protein synthesis II. A convenient assay for the ribosome-dependent synthesis of N-formyl-C14-methionylpuromycin.蛋白质合成的起始II。一种用于核糖体依赖性合成N-甲酰基-C14-甲硫氨酰嘌呤霉素的简便测定法。

Biochem Biophys Res Commun. 1966 Oct 20;25(2):233-8. doi: 10.1016/0006-291x(66)90586-9.

7

Regulatory mechanisms and protein synthesis. X. Codon recognition on 30 S ribosomes.调控机制与蛋白质合成。十、30 S核糖体上的密码子识别

J Mol Biol. 1966 Oct 28;21(1):145-71. doi: 10.1016/0022-2836(66)90085-4.

8

Polypeptidyl-sigma-ribonucleic acid and amino-acyl-sigma-ribonucleic acid binding sites on ribosomes.核糖体上的多肽基-σ-核糖核酸和氨酰基-σ-核糖核酸结合位点。

Nature. 1966 Jul 23;211(5047):380-4. doi: 10.1038/211380a0.

9

The mechanism of messenger RNA translocation through ribosomes.信使核糖核酸通过核糖体的转运机制。

Proc Natl Acad Sci U S A. 1969 Sep;64(1):388-95. doi: 10.1073/pnas.64.1.388.

10

Deacylated tRNA-phe binding to a reticulocyte ribosomal site for the initiation of polyphenylalanine synthesis.脱酰基苯丙氨酸转运核糖核酸与网织红细胞核糖体位点结合以起始多聚苯丙氨酸合成。

Proc Natl Acad Sci U S A. 1969 Aug;63(4):1431-8. doi: 10.1073/pnas.63.4.1431.

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