Russell S M
Department of Poultry Science, The University of Georgia, Athens 30602-2772, USA.
J Food Prot. 2000 Sep;63(9):1179-83. doi: 10.4315/0362-028x-63.9.1179.
A study was conducted to compare commonly used methods, such as Petrifilm and SimPlate, and the rapid microbiological methods BioSys optical and Bactometer conductance to the standard most probable number (MPN) procedure for enumerating Escherichia coli from poultry carcasses and ground beef. Broiler carcasses and ground beef were evaluated in each of three replicate trials. Five groups of carcasses or ground beef were sampled and analyzed using Petrifilm, SimPlate, BioSys optical, and Bactometer conductance measurements after temperature abuse at 37 degrees C for 0 (Petrifilm and SimPlate only), 2, 4, 6, or 8 h. The correlation coefficients for the regression lines comparing the standard E. coli MPN procedure to Petrifilm and SimPlate for chicken and ground beef, respectively, were as follows: 0.95, 0.94, 0.93, and 0.91. The correlation coefficients for the regression lines comparing the standard E. coli MPN procedure to BioSys optical and Bactometer conductance measurements for chicken and ground beef, respectively, were -0.91, -0.90, -0.93, and -0.96. Although Petrifilm and SimPlate performed well, E. coli could not be enumerated from 16.7 and 10% of samples, respectively, using these methods. The BioSys optical and Bactometer conductance methods performed very well when compared with Petrifilm and SimPlate. Using rapid methods (BioSys optical and Bactometer conductance), results were obtained in 1 to 11 h rather than the 48 h required to conduct Petrifilm or SimPlate or the 5 days required to conduct the MPN procedure. These methods may allow processors to test products and obtain results before shipping, avoiding the cost and loss of reputation associated with a recall or foodborne illness outbreak.
开展了一项研究，以比较常用方法（如Petrifilm和SimPlate）以及快速微生物检测方法BioSys光学法和Bactometer电导法与用于从家禽胴体和碎牛肉中计数大肠杆菌的标准最大可能数（MPN）程序。在三个重复试验中分别对肉鸡胴体和碎牛肉进行了评估。对五组胴体或碎牛肉进行采样，并在37℃温度滥用0小时（仅Petrifilm和SimPlate）、2小时、4小时、6小时或8小时后，使用Petrifilm、SimPlate、BioSys光学法和Bactometer电导法进行分析。将标准大肠杆菌MPN程序分别与用于鸡肉和碎牛肉的Petrifilm和SimPlate进行比较的回归线的相关系数如下：0.95、0.94、0.93和0.91。将标准大肠杆菌MPN程序分别与用于鸡肉和碎牛肉的BioSys光学法和Bactometer电导法进行比较的回归线的相关系数分别为-0.91、-0.90、-0.93和-0.96。尽管Petrifilm和SimPlate表现良好，但使用这些方法分别无法从16.7%和10%的样品中计数大肠杆菌。与Petrifilm和SimPlate相比，BioSys光学法和Bactometer电导法表现非常好。使用快速方法（BioSys光学法和Bactometer电导法），可在1至11小时内获得结果，而不是进行Petrifilm或SimPlate所需的48小时或进行MPN程序所需的5天。这些方法可能使加工商能够在发货前对产品进行检测并获得结果，避免与召回或食源性疾病爆发相关的成本和声誉损失。
