Chenite A, Chaput C, Wang D, Combes C, Buschmann M D, Hoemann C D, Leroux J C, Atkinson B L, Binette F, Selmani A
Center for Applied Research on Polymers, Chemical Engineering Department and Institute of Biomedical Engineering, Ecole Polytechnique of Montreal, PQ, Canada.
Biomaterials. 2000 Nov;21(21):2155-61. doi: 10.1016/s0142-9612(00)00116-2.
A novel approach to provide, thermally sensitive neutral solutions based on chitosan/polyol salt combinations is described. These formulations possess a physiological pH and can be held liquid below room temperature for encapsulating living cells and therapeutic proteins; they form monolithic gels at body temperature. When injected in vivo the liquid formulations turn into gel implants in situ. This system was used successfully to deliver biologically active growth factors in vivo as well as an encapsulating matrix for living chondrocytes for tissue engineering applications. This study reports for the first time the use of polymer/polyol salt aqueous solutions as gelling systems, suggesting the discovery of a prototype for a new family of thermosetting gels highly compatible with biological compounds.
本文描述了一种基于壳聚糖/多元醇盐组合提供热敏中性溶液的新方法。这些制剂具有生理pH值，在室温以下可保持液态以封装活细胞和治疗性蛋白质；它们在体温下形成整体凝胶。当体内注射时，液体制剂原位转变为凝胶植入物。该系统已成功用于在体内递送生物活性生长因子，以及作为用于组织工程应用的活软骨细胞的封装基质。本研究首次报道了使用聚合物/多元醇盐水溶液作为凝胶系统，这表明发现了一种与生物化合物高度相容的新型热固性凝胶家族的原型。
