Carbajal D, Molina V, Noa M, Valdés S, Arruzazabala M L, Aguilar C, Más R
Department of Pharmacology, Center of Natural Products, National Center of Scientific Research, Ciudad Habana, Cuba.
Pharmacol Res. 2000 Oct;42(4):329-32. doi: 10.1006/phrs.2000.0693.
This study was designed to determine the effect of D-002, a natural product isolated and purified from beeswax (Apis mellifera), on gastric mucus composition on ethanol-induced ulcer in rats. The morphology of the lesions was analysed histologically, and morphometric analysis of gastric-gland content in total glycoprotein and sulphated macromolecules were done. Oral pretreatment with D-002 at 5 and 25 mgkg(-1)1 before oral administration of ethanol at 60%, produced a significant increase in the amount of gastric mucus and total protein. The histomorphometric evaluation of the gastric damage at the same doses showed a significant increase in neutral glycoproteins and sulfated macromolecules. It is concluded that enhancement of the quantity and quality of the mucus could partly explain the gastroprotective effect of D-002.
本研究旨在确定从蜂蜡（意大利蜜蜂）中分离纯化得到的天然产物D - 002对大鼠乙醇诱导型溃疡胃黏液成分的影响。对病变形态进行了组织学分析，并对胃腺中总糖蛋白和硫酸化大分子进行了形态计量分析。在口服60%乙醇之前，以5和25 mgkg(-1)1的剂量对大鼠进行D - 002口服预处理，可使胃黏液和总蛋白量显著增加。相同剂量下对胃损伤的组织形态计量学评估显示中性糖蛋白和硫酸化大分子显著增加。结论是，黏液数量和质量的增强可能部分解释了D - 002的胃保护作用。
