Departamento de Anatomia, Biologia Celular, Fisiologia e Biofísica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil.
J Ethnopharmacol. 2011 Sep 1;137(1):192-8. doi: 10.1016/j.jep.2011.05.006. Epub 2011 May 11.
Indigofera suffruticosa is specie typical of the "Cerrado" or Brazilian savannah; it is a member of the Fabaceae family - in folkmedicine is used for gastric disorders, infection and inflammation.
Ethyl acetate fraction (AcF) and aqueous fraction (AqF) of the methanolic extract of I. suffruticosa leaves were evaluated against acute gastric ulcer. The AcF fraction was selected to assess its activity in ulcer healing and its gastroprotective effects via mucus and gastric secretion.
The gastroprotective action of AcF and AqF fractions were evaluated in a rodent experimental model. The action mechanisms, involvements of the antisecretory action, mucus and prostaglandin production, toxicological and healing activity of the AcF (100mg/kg, p.o.) were evaluated. We also used histological analysis (HE and PAS) and immunohistochemical (PCNA and HSP-70) assays to evaluate the effects of I. suffruticosa.
AcF significantly inhibited the gastric mucosal damage caused by ethanol. This effect was statistically significant in 100mg/kg group compared vehicle. AcF did not interfered with gastric secretion, significantly increased the PGE(2) and mucus production (validated in PAS technique). The gastroprotection was attenuated by pretreatment with N-ethylmaleimide, but not L-NAME. In acid-acetic-induced ulcer model AcF accelerated ulcer healing. Immunohistochemistry analysis showed induction of proliferating cell (PCNA) and heat shock protein (HSP 70).
These results showed that AcF acted as gastroprotective agent stimulating prostaglandin, mucus and HSP70.
木蓝是“塞拉多”或巴西热带稀树草原特有的物种；它是豆科植物的一员——在民间医学中，用于治疗胃病、感染和炎症。
木蓝叶甲醇提取物的乙酸乙酯部分（AcF）和水部分（AqF）被评估用于治疗急性胃溃疡。选择 AcF 部分评估其在溃疡愈合中的活性及其通过粘液和胃分泌的胃保护作用。
在啮齿动物实验模型中评估 AcF 和 AqF 部分的胃保护作用。评估 AcF（100mg/kg，po）的作用机制、涉及的抗分泌作用、粘液和前列腺素产生、毒理学和愈合活性。我们还使用组织学分析（HE 和 PAS）和免疫组织化学（PCNA 和 HSP-70）分析来评估木蓝的作用。
AcF 显著抑制了乙醇引起的胃粘膜损伤。与载体相比，100mg/kg 组的这种效果具有统计学意义。AcF 不干扰胃分泌，显著增加 PGE（2）和粘液产生（在 PAS 技术中得到验证）。用 N-乙基马来酰亚胺预处理可减弱胃保护作用，但 L-NAME 不行。在酸-乙酸诱导的溃疡模型中，AcF 加速了溃疡愈合。免疫组织化学分析显示增殖细胞（PCNA）和热休克蛋白（HSP 70）的诱导。
这些结果表明 AcF 作为胃保护剂，刺激前列腺素、粘液和 HSP70。
