Keller C A, Glasmästar K, Zhdanov V P, Kasemo B
Department of Applied Physics, Chalmers University of Technology, S-412 96 Göteborg, Sweden.
Phys Rev Lett. 2000 Jun 5;84(23):5443-6. doi: 10.1103/PhysRevLett.84.5443.
Using a combination of the quartz crystal microbalance and surface plasmon resonance techniques, we have studied the spontaneous formation of supported lipid bilayers from small (approximately 25 nm) unilamellar vesicles. Together these experimental methods measure the amount of lipid adsorbed on the surface and the amount of water trapped by the lipid. With this approach, we have, for the first time, been able to observe in detail the progression from the adsorption of intact vesicles to rupture and bilayer formation. Monte Carlo simulations reproduce the data.
通过结合石英晶体微天平与表面等离子体共振技术，我们研究了由小（约25纳米）单层囊泡自发形成支撑脂质双层的过程。这些实验方法共同测量了吸附在表面的脂质数量以及被脂质捕获的水量。通过这种方法，我们首次得以详细观察从完整囊泡的吸附到破裂及双层形成的过程。蒙特卡罗模拟重现了这些数据。
