Villa L, Pezzella C, Tosini F, Visca P, Petrucca A, Carattoli A
Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Rome, Italy.
Antimicrob Agents Chemother. 2000 Oct;44(10):2911-4. doi: 10.1128/AAC.44.10.2911-2914.2000.
A conjugative IncL/M plasmid (pSEM) conferring resistance to gentamicin, amikacin, kanamycin, sulfonamides, and expanded-spectrum cephalosporins was found in pathogenic strains of Salmonella enterica serotype Typhimurium. Resistance to aminoglycosides was encoded by a sul1-type class 1 integron (In-t3). An extended-spectrum beta-lactamase gene, bla(SHV-5), was identified 3. 5 kb downstream of the integrase (intI1) gene of In-t3. Nucleotide sequence analysis of the 5.3-kb bla(SHV-5)-In-t3 region of pSEM highlighted striking similarities with IncL/M plasmids isolated from nosocomial gram-negative pathogens, conferring resistance to expanded-spectrum cephalosporins and aminoglycosides.
在肠炎沙门氏菌鼠伤寒血清型的致病菌株中发现了一种接合型IncL/M质粒（pSEM），它赋予对庆大霉素、阿米卡星、卡那霉素、磺胺类药物和广谱头孢菌素的抗性。对氨基糖苷类的抗性由一个sul1型1类整合子（In-t3）编码。在In-t3整合酶（intI1）基因下游3.5 kb处鉴定出一个超广谱β-内酰胺酶基因bla(SHV-5)。对pSEM的5.3 kb bla(SHV-5)-In-t3区域的核苷酸序列分析突出显示了与从医院革兰氏阴性病原体分离出的IncL/M质粒有显著相似性，这些质粒赋予对广谱头孢菌素和氨基糖苷类的抗性。
