Ekerhovd E, Brännström M, Weijdegârd B, Norström A
Department of Obstetrics and Gynecology, Göteborg University, Göteborg, Sweden.
Am J Obstet Gynecol. 2000 Sep;183(3):610-6. doi: 10.1067/mob.2000.105901.
The purpose of the study was to determine whether a nitric oxide-generating system exists in the uterine cervix at term pregnancy and to study the effects of nitric oxide on contracting cervical strips.
Tissue specimens were obtained from the cervices of women after deliveries and at elective cesarean deliveries. Immunohistochemical techniques and immunoblotting were used to identify isoforms of nitric oxide synthase. The effects of nitric oxide on cervical contractility were examined by the addition of nitroglycerin or spermine NONOate [(Z)-1-(N -[3-aminopropyl]-N -[4-(3-aminopropyl-ammonio)butyl]-amino)-diazen-1-ium-1,2-++ +diolate] to organ baths.
Immunohistochemical examination demonstrated positive staining for both endothelial and inducible nitric oxide synthase. Both isoforms of nitric oxide synthase were clearly detectable by immunoblotting. Significant inhibition of contractile activity (10(-7)-10(-5) mol/L) was observed when nitroglycerin or spermine NONOate was administered.
An endogenous nitric oxide system is present in the uterine cervix at term, and this tissue is responsive to nitric oxide, which causes relaxation of the cervical muscle.
本研究旨在确定足月妊娠时子宫颈是否存在一氧化氮生成系统，并研究一氧化氮对宫颈条收缩的影响。
组织标本取自分娩后及择期剖宫产妇女的宫颈。采用免疫组织化学技术和免疫印迹法鉴定一氧化氮合酶的同工型。通过向器官浴中添加硝酸甘油或精胺NONOate[（Z）-1-（N-[3-氨基丙基]-N-[4-（3-氨基丙基铵基）丁基]-氨基）-重氮-1-鎓-1,2-二醇盐]来检测一氧化氮对宫颈收缩性的影响。
免疫组织化学检查显示内皮型和诱导型一氧化氮合酶均呈阳性染色。通过免疫印迹法可清楚检测到两种一氧化氮合酶同工型。给予硝酸甘油或精胺NONOate时，观察到收缩活性受到显著抑制（10⁻⁷ - 10⁻⁵mol/L）。
足月时子宫颈存在内源性一氧化氮系统，该组织对一氧化氮有反应，一氧化氮可使宫颈肌肉松弛。
