Alasehirli Belgin, Cekmen Mustafa, Nacak Muradiye, Balat Ayse
Department of Pharmacology, Gaziantep University Medical Faculty, Gaziantep, Turkey.
Department of Biochemistry, Kocaeli University Medical Faculty, Kocaeli, Turkey.
Curr Ther Res Clin Exp. 2005 Mar;66(2):130-7. doi: 10.1016/j.curtheres.2005.04.003.
Nitric oxide (NO) is a potent vasodilator that might have an10 important role in the modulation of maternal and fetal vascular tone during pregnancy. The effects of caffeine intake on maternal and fetal hemodynamic properties during pregnancy have been investigated in several human and animal studies. However, based on a literature search, there has been no study of placental total nitrite (a stable product of NO) concentration (PTNC) in pregnant humans or rats given caffeine.
The aim of this study was to assess the effects of caffeine intake 10 on PTNC in rats.
This 21-day, vehicle-controlled study was conducted at the Department10 of Pharmacology, Gaziantep University Hospital, Gaziantep, Turkey. Female Wistar rats were randomly assigned; based on age and weight, to receive 25, 50, or 100 mg/kg QD caffeine or 50 mg/kg QD isotonic saline solution (vehicle; age-matched control group), intraperitoneally for 21 days. After euthanization of the rats and cesarean section, the numbers of fetuses and fetal deaths were counted. The lengths and weights of the fetuses in each study group were noted. PTNC in the rats was determined using the Greiss reaction.
This study included 26 rats (7, 7, and 6 rats in the groups receiving10 25, 50, and 100 mg/kg. d caffeine, respectively; 6 rats in the control group). The mean (SD) lengths of the fetuses of the rats given 25, 50, and 100 mg/kg · d caffeine (4.90 [0.15], 4.02 [0.27], and 3.45 [0.17] mm, respectively) were significantly less compared with controls (5.10 [0.18] mm) (all, P < 0.001), as were the mean (SD) weights of the fetuses of rats given caffeine (5.86 [0.24], 4.97 [0.59], and 3.41 [0.23] g, respectively) versus controls (6.18 [0.21] g) (all, P < 0.001). The mean (SD) PTNCs in rats given 25, 50, and 100 mg/kg. d caffeine (19.82 [1.97], 29.39 [2.07], and 45.51 [7.66] nmol/g, respectively) were significantly higher compared with controls (16.10 [2.12] nmol/g) (all, P < 0.001).
The results of this study in rats suggest that caffeine intake 10 might increase NO production in the placenta. In addition, based on our findings and those from previous studies, we suggest that this increase might be an adaptive physiologic response to prevent undesirable effects of caffeine on vascular tone during pregnancy.
一氧化氮（NO）是一种强效血管舒张剂，可能在孕期母体和胎儿血管张力调节中发挥重要作用。多项人体和动物研究探讨了孕期摄入咖啡因对母体和胎儿血液动力学特性的影响。然而，经文献检索发现，尚无关于给予咖啡因的孕鼠或孕妇胎盘总亚硝酸盐（NO的稳定产物）浓度（PTNC）的研究。
本研究旨在评估咖啡因摄入对大鼠PTNC的影响。
本为期21天的载体对照研究在土耳其加济安泰普大学医院药理学系开展。雌性Wistar大鼠按年龄和体重随机分组，分别腹腔注射25、50或100mg/kg每日一次的咖啡因或50mg/kg每日一次的等渗盐溶液（载体；年龄匹配对照组），持续21天。大鼠安乐死后行剖宫产，统计胎儿数量及胎儿死亡情况。记录各研究组胎儿的长度和体重。采用格里斯反应测定大鼠的PTNC。
本研究纳入26只大鼠（分别给予25、50和100mg/kg·d咖啡因的组中各有7、7和6只大鼠；对照组6只大鼠）。给予25、50和100mg/kg·d咖啡因的大鼠胎儿平均（标准差）长度（分别为4.90[0.15]、4.02[0.27]和3.45[0.17]mm）显著短于对照组（5.10[0.18]mm）（均P<0.001），给予咖啡因的大鼠胎儿平均（标准差）体重（分别为5.86[0.24]、4.97[0.59]和3.41[0.23]g）也显著低于对照组（6.18[0.
