Sohn H Y, Murray D B, Kuriyama H
Biochemical Engineering Laboratory, National Institute of Bioscience and Human Technology, 1-1, Higashi. Tsukuba, Ibaraki 305-8566, Japan.
Yeast. 2000 Sep 30;16(13):1185-90. doi: 10.1002/1097-0061(20000930)16:13<1185::AID-YEA619>3.0.CO;2-W.
Saccharomyces cerevisiae showed an ultradian respiratory oscillation during aerobic continuous culture. Analysis of the off-gas revealed that hydrogen sulphide production also oscillated. Production was first detected at the onset of low respiration and reached a maximum (1.5 microM) prior to minimum respiratory activity. Then H(2)S concentration fell rapidly to below 0.2 microM before the onset of high respiration. Injection of respiratory oscillation perturbation agents, such as glutathione (50 microM), NaNO(2) (50 microM) or acetaldehyde (4.5 mM),() transiently increased H(2)S production above 6 microM. The synchronization properties of H(2)S were analysed to reveal that changes of oscillation period and amplitude were dependent on H(2)S concentration in culture. It is concluded that H(2)S produced during oscillation produces population synchrony by respiratory chain inhibition.
酿酒酵母在需氧连续培养过程中呈现出超日呼吸振荡。废气分析表明，硫化氢的产生也存在振荡。在低呼吸开始时首次检测到硫化氢的产生，并且在最低呼吸活性之前达到最大值（1.5微摩尔）。然后，在高呼吸开始之前，硫化氢浓度迅速降至0.2微摩尔以下。注入呼吸振荡扰动剂，如谷胱甘肽（50微摩尔）、亚硝酸钠（50微摩尔）或乙醛（4.5毫摩尔），会使硫化氢产量短暂增加至6微摩尔以上。对硫化氢的同步特性进行分析后发现，振荡周期和幅度的变化取决于培养物中硫化氢的浓度。得出的结论是，振荡过程中产生的硫化氢通过抑制呼吸链产生群体同步性。
