Pansarasa O, Castagna L, Colombi B, Vecchiet J, Felzani G, Marzatico F
Department of Physiological and Pharmacological Sciences, University of Pavia, Italy.
Free Radic Res. 2000 Sep;33(3):287-93. doi: 10.1080/10715760000301451.
Previous studies, conducted on experimental animals, have indicated that reactive oxygen species (ROS) are involved in the aging process. The objective of this work was to study the relationship between oxidative damage and human skeletal muscle aging, measuring the activity of the main antioxidant enzymes superoxide dismutase (total and MnSOD), glutathione peroxidase (GPx) and catalase in the skeletal muscle of men and women in the age groups: young (17-40 years), adult (41-65 years) and aged (66-91 years). We also measured glutathione and glutathione disulfide (GSH and GSSG) levels and the redox index; lipid peroxidation and protein carbonyl content. Total SOD activity was lower in the 66-91 year-old vs. the 17-40 year-old men; MnSOD activity was significantly greater in 66-91 year-old vs. 17-40 year-old women. GPx activity remained unchanged. The activity of catalase was lower in adults than in young men but higher in the aged. We observed no changes in GSH levels and significantly higher GSSG levels only in aged men vs. adult men, and a significant decrease in aged women vs. aged men. The protein carbonyl content increased significantly in the 41-65 and 66-91 year-old vs. the 17-40 year-old men. Finally, young women have lower lipid peroxidation levels than young men. Significantly higher lipid peroxidation levels were observed in aged men vs. both young and adult men, and the same trend was noticed for women. We conclude that oxidative damage may play a crucial role in the decline of functional activity in human skeletal muscle with normal aging in both sexes; and that men appear to be more subject to oxidative stress than women.
先前对实验动物进行的研究表明，活性氧（ROS）参与衰老过程。这项工作的目的是研究氧化损伤与人类骨骼肌衰老之间的关系，测量不同年龄组男性和女性骨骼肌中主要抗氧化酶超氧化物歧化酶（总超氧化物歧化酶和锰超氧化物歧化酶）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶的活性。我们还测量了谷胱甘肽和谷胱甘肽二硫化物（GSH和GSSG）水平以及氧化还原指数；脂质过氧化和蛋白质羰基含量。66 - 91岁男性的总超氧化物歧化酶活性低于17 - 40岁男性；66 - 91岁女性的锰超氧化物歧化酶活性显著高于17 - 40岁女性。GPx活性保持不变。成年人的过氧化氢酶活性低于年轻男性，但高于老年人。我们观察到GSH水平没有变化，仅老年男性与成年男性相比GSSG水平显著升高，老年女性与老年男性相比GSSG水平显著降低。41 - 65岁和66 - 91岁男性的蛋白质羰基含量相对于17 - 40岁男性显著增加。最后，年轻女性的脂质过氧化水平低于年轻男性。老年男性与年轻男性和成年男性相比，脂质过氧化水平均显著更高，女性也呈现相同趋势。我们得出结论，氧化损伤可能在男女正常衰老过程中人类骨骼肌功能活动下降中起关键作用；而且男性似乎比女性更容易受到氧化应激的影响。
