Crowther R A, Daniel S E, Goedert M
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Neurosci Lett. 2000 Oct 6;292(2):128-30. doi: 10.1016/s0304-3940(00)01440-3.
The defining neuropathological deposits of Parkinson's disease, dementia with Lewy bodies and multiple system atrophy are strongly immunoreactive for alpha-synuclein. We have shown previously that isolated filaments from dementia with Lewy bodies and multiple system atrophy brains are labelled in a characteristic fashion by a number of alpha-synuclein antibodies. Here we have extracted filaments from substantia nigra of patients with idiopathic Parkinson's disease. Antibodies directed against the carboxy-terminal region of alpha-synuclein labelled isolated filaments along their entire lengths. By contrast, an antibody directed against the amino-terminal region of alpha-synuclein only labelled one filament end. These characteristics were identical to those of filaments extracted from brains of patients with dementia with Lewy bodies and multiple system atrophy.
帕金森病、路易体痴呆和多系统萎缩的典型神经病理沉积物对α-突触核蛋白具有强烈的免疫反应性。我们之前已经表明，从路易体痴呆和多系统萎缩脑部分离出的细丝被多种α-突触核蛋白抗体以一种特征性方式标记。在此，我们从特发性帕金森病患者的黑质中提取了细丝。针对α-突触核蛋白羧基末端区域的抗体沿着分离出的细丝全长进行标记。相比之下，一种针对α-突触核蛋白氨基末端区域的抗体仅标记细丝的一端。这些特征与从路易体痴呆和多系统萎缩患者大脑中提取的细丝的特征相同。
