Evans G, Roversi P, Bricogne G
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, England.
Acta Crystallogr D Biol Crystallogr. 2000 Oct;56(Pt 10):1304-11. doi: 10.1107/s0907444900008982.
It is demonstrated that standard in-house protein crystal X-ray diffraction apparatus can be used to measure very low resolution reflections with only a few modifications. The apparatus and modifications are described in detail and tested on two different macromolecular crystal samples: lysozyme and the 30S ribosomal subunit. Contrast-variation measurements on tetragonal hen egg-white lysozyme demonstrate the potential usefulness of the apparatus in providing accurate data for the determination of macromolecular envelopes. In contrast, the measurement of very low resolution diffraction from crystals of the 30S ribosome subunit illustrates how in-house facilities can provide data from small weakly diffracting crystals of a very large macromolecule.
结果表明，只需进行一些修改，标准的内部蛋白质晶体X射线衍射仪就可用于测量极低分辨率的反射。详细描述了该仪器及其修改方法，并在两种不同的大分子晶体样品上进行了测试：溶菌酶和30S核糖体亚基。对四方晶系的鸡蛋清溶菌酶进行的对比变化测量证明了该仪器在为确定大分子包膜提供准确数据方面的潜在用途。相比之下，对30S核糖体亚基晶体的极低分辨率衍射测量说明了内部设备如何能够从非常大的大分子的小的弱衍射晶体中提供数据。
