Chu P H, Bardwell W M, Gu Y, Ross J, Chen J
Department of Medicine, School of Medicine, University of California at San Diego, La Jolla, California 92093-0613, USA.
Mol Cell Biol. 2000 Oct;20(20):7460-2. doi: 10.1128/MCB.20.20.7460-7462.2000.
LIM domain-containing proteins play critical roles in vertebrate development and cellular differentiation. Recently, four members of the four and one-half LIM protein (FHL) family have been identified and cloned. One of these, FHL2, is expressed in a restricted manner in the cardiovascular system throughout development into adulthood, suggesting that FHL2 may play an important role in cardiovascular development and function. Here we describe the generation and analysis of mice carrying a null mutation of the FHL2 gene. FHL2-deficient mice are viable and maintain normal cardiac function both before and after acute mechanical stress induced by aortic constriction. These data suggest that FHL2 is not essential for cardiac development and function.
含LIM结构域的蛋白质在脊椎动物发育和细胞分化过程中发挥着关键作用。最近，已鉴定并克隆出四又二分之一LIM蛋白（FHL）家族的四个成员。其中之一，即FHL2，在整个发育至成年阶段的心血管系统中呈局限性表达，这表明FHL2可能在心血管发育和功能中发挥重要作用。在此，我们描述了携带FHL2基因无效突变的小鼠的产生及分析情况。FHL2基因缺陷型小鼠能够存活，并且在主动脉缩窄诱导的急性机械应激前后均维持正常心脏功能。这些数据表明，FHL2对于心脏发育和功能并非必不可少。
