Calvo J L, Boya J, Carbonell A L, García-Mauriño J E
Department of Histology, Faculty of Medicine, Complutense University, Madrid, Spain.
Histol Histopathol. 2000 Oct;15(4):1005-10. doi: 10.14670/HH-15.1005.
The immunohistochemical detection of bromodeoxyuridine (BrdU) was used to study the cell proliferation in the developing rat pineal gland, from the appearance of pineal primordium in the embryonic day 15 (E15) until 30 days after birth. The results showed three different proliferative phases. From E15 to E21, the pineal gland shows a phase of rapid proliferation. The second phase corresponds to the first postnatal week, in which the number of labeled cells per surface unit decreases suddenly to values between 20% to 10% of those of embryonic period. From the second postnatal week onwards, the number of BrdU-positive cells progressively decreases.
采用免疫组化法检测5-溴脱氧尿嘧啶核苷（BrdU），以研究发育中大鼠松果体从胚胎第15天（E15）出现松果体原基直至出生后30天的细胞增殖情况。结果显示出三个不同的增殖阶段。从E15到E21，松果体呈现快速增殖阶段。第二阶段对应出生后的第一周，在此期间，每单位表面积的标记细胞数量突然降至胚胎期的20%至10%之间。从出生后第二周起，BrdU阳性细胞数量逐渐减少。
