Carvajal J C, Carbajo S, Gómez Esteban M B, Alvarez-Morujo Suárez A J, Muñoz Barragán L
Department of Human Anatomy and Histology, Faculty of Medicine, Universidad de Salamanca, Spain.
Histol Histopathol. 1998 Jul;13(3):697-701. doi: 10.14670/HH-13.697.
To establish a possible correlation between the rate of cellular proliferation and already documented functional and morphological characteristics of the rat pineal gland during postnatal development, the bromodeoxyuridine labelling method was used to evaluate the fraction of cells at the S phase of the cell cycle in paraffin sections from 1-, 7-, 14- and 28-day-old rats. Numerical density, taken as an indirect measure of cell hypertrophy, was also evaluated. During the first week after birth the percentage of S phase-cells in the rat pineal gland sharply decreased from around 9% to 1.3%. A smaller but also significant decrease was found from the 7th to the 14th postnatal day where S phase cells were less than 0.5% of all pineal cells. A very low percentage was also seen in samples from 28-day-old rats. Numerical density, namely, the total number of cells per surface unit of pineal section, decreased from birth to the end of the first month. This decrease was also steeper from birth to the 7th postnatal day than at any other period of the study. These results support the idea that a strong expansion of the cellular population of the rat pineal gland precedes morphological and functional maturation and opens the way to further exploration of the relationship between functional and proliferative responses of the pineal gland.
为了确定细胞增殖速率与出生后发育过程中大鼠松果体已记录的功能和形态特征之间可能存在的相关性，采用溴脱氧尿苷标记法评估1日龄、7日龄、14日龄和28日龄大鼠石蜡切片中处于细胞周期S期的细胞比例。还评估了作为细胞肥大间接指标的数值密度。出生后的第一周内，大鼠松果体中S期细胞的百分比从约9%急剧下降至1.3%。在出生后第7天至第14天也发现了较小但同样显著的下降，此时S期细胞占所有松果体细胞的比例不到0.5%。在28日龄大鼠的样本中也观察到了非常低的百分比。数值密度，即松果体切片每单位面积的细胞总数，从出生到第一个月末有所下降。从出生到出生后第7天的下降也比研究中的任何其他时期更为明显。这些结果支持了这样一种观点，即大鼠松果体细胞群的强烈扩张先于形态和功能成熟，并为进一步探索松果体功能和增殖反应之间的关系开辟了道路。
