Gariano R F, Nath A K, D'Amico D J, Lee T, Sierra-Honigmann M R
Departments of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, Connecticut 06520-8061, USA.
Invest Ophthalmol Vis Sci. 2000 Oct;41(11):3576-81.
Leptin is a cytokine that regulates energy metabolism and is linked to diabetes mellitus through its metabolic actions. Leptin is angiogenic and promotes wound healing, and therefore this investigation was conducted to determine whether leptin is associated with neovascular and fibrotic complications of diabetes and other retinopathies.
Serum and vitreous samples were collected from patients classified by the presence and type of diabetic retinopathy or other ocular diseases. Leptin was measured in serum and vitreous by radioimmunoassay, and leptin and leptin receptor were localized in epiretinal membranes immunohistochemically.
Leptin levels in serum and vitreous were higher in patients with diabetes than in those without, and vitreous leptin concentrations were especially elevated in patients with proliferative diabetic retinopathy or retinal detachment. Leptin and leptin receptor were detected in fibrovascular epiretinal membrane of patients with diabetes.
Leptin in human vitreous is elevated in proliferative diabetic retinopathy, and retinal detachment and is present in fibrovascular epiretinal tissue. These data suggest an involvement of leptin in retinal disease.
瘦素是一种调节能量代谢的细胞因子，通过其代谢作用与糖尿病相关联。瘦素具有促血管生成作用并能促进伤口愈合，因此开展本研究以确定瘦素是否与糖尿病及其他视网膜病变的新生血管和纤维化并发症有关。
收集根据糖尿病视网膜病变或其他眼部疾病的存在情况及类型进行分类的患者的血清和玻璃体样本。通过放射免疫分析法测定血清和玻璃体中的瘦素水平，并采用免疫组织化学方法在视网膜前膜中定位瘦素和瘦素受体。
糖尿病患者血清和玻璃体中的瘦素水平高于非糖尿病患者，增殖性糖尿病视网膜病变或视网膜脱离患者的玻璃体瘦素浓度尤其升高。在糖尿病患者的纤维血管性视网膜前膜中检测到瘦素和瘦素受体。
在增殖性糖尿病视网膜病变和视网膜脱离患者中，人玻璃体中的瘦素水平升高，且存在于纤维血管性视网膜前组织中。这些数据表明瘦素参与了视网膜疾病。
