Lewis V J, Walter P D, Thacker W L, Winkler W G
J Clin Microbiol. 1975 Sep;2(3):193-7. doi: 10.1128/jcm.2.3.193-197.1975.
Levels of lymphocytic choriomeningitis virus antibody were assayed in 62 infected persons. The three tests used were indirect fluorescent antibody (IFA), complement fixation, and neutralization in mice. The sera first became positive by the IFA test, and IFA titers rapidly rose to a relatively high level, with the sera remaining positive long after the antibody detectable by complement fixation had disappeared. The IFA test appeared to be specific. The sera became positive last by the mouse neutralization test; with this test, antibody first appeared several weeks after infection. Virus-infected cells were stable when stored at -60 C, allowing diagnostic sera to be tested promptly by the IFA test. The IFA test for lymphocytic choriomeningitis antibody should increase the number of serological diagnoses, since it is not only rapid and specific, but detects cases not diagnosed by the other methods.
对62名感染者进行了淋巴细胞性脉络丛脑膜炎病毒抗体水平检测。所采用的三项检测分别为间接荧光抗体法（IFA）、补体结合试验和小鼠中和试验。血清首先通过IFA检测呈阳性，IFA滴度迅速升至相对较高水平，在补体结合试验可检测到的抗体消失很久之后，血清仍保持阳性。IFA检测似乎具有特异性。血清最后通过小鼠中和试验呈阳性；通过该试验，抗体在感染后数周才首次出现。病毒感染细胞在-60℃保存时稳定，这使得诊断血清能够通过IFA检测迅速进行检测。淋巴细胞性脉络丛脑膜炎抗体的IFA检测应能增加血清学诊断的数量，因为它不仅快速、特异，而且能检测出其他方法未诊断出的病例。
