一种用于测定对淋巴细胞性脉络丛脑膜炎病毒中和抗体反应的快速荧光灶抑制试验。

A rapid fluorescent focus-inhibition test for determining the neutralizing-antibody response to lymphocytic choriomeningitis virus.

作者信息

Thacker W L, Lewis V J, Haller G J, Baer G M

出版信息

Can J Microbiol. 1977 May;23(5):522-6. doi: 10.1139/m77-077.

Abstract

Levels of neutralizing antibody to lymphocytic choriomeningitis (LCM) virus in the sera of 66 infected persons were assayed by a rapid fluorescent focus-inhibition test (RFFIT). The test was more sensitive than the mouse-neutralization (MN) test and could be completed in less than 24 h. The RFFIT titers were compared with titers obtained by the indirect fluorescent-antibody (IFA) and complement-fixation (CF) tests. Neutralizing antibody detected by the RFFIT remained positive after IRA, CF and MN antibodies had disappeared. The RFFIT for detection of LCM antibody is specific and reproducible and seems especially useful for determining the incidence and epidemiology of LCM virus infections.

摘要

采用快速荧光灶抑制试验（RFFIT）检测了66例感染者血清中针对淋巴细胞性脉络丛脑膜炎（LCM）病毒的中和抗体水平。该试验比小鼠中和试验（MN）更灵敏，且可在24小时内完成。将RFFIT滴度与间接荧光抗体（IFA）试验和补体结合（CF）试验所获滴度进行了比较。在免疫反应性抗体（IRA）、CF抗体和MN抗体消失后，RFFIT检测到的中和抗体仍呈阳性。用于检测LCM抗体的RFFIT具有特异性和可重复性，似乎对确定LCM病毒感染的发病率和流行病学情况特别有用。