运用联合显微放射自显影术和荧光原位杂交技术来测定无色菌属未培养细菌混合自然群落中的碳代谢。
Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium.
作者信息
Gray N D, Howarth R, Pickup R W, Jones J G, Head I M
机构信息
Fossil Fuels and Environmental Geochemistry Postgraduate Institute and Centre for Molecular Ecology, University of Newcastle, Newcastle upon Tyne NE1 7RU, United Kingdom.
出版信息
Appl Environ Microbiol. 2000 Oct;66(10):4518-22. doi: 10.1128/AEM.66.10.4518-4522.2000.
Abstract
Combined microautoradiography and fluorescence in situ hybridization (FISH) was used to investigate carbon metabolism in uncultured bacteria from the genus Achromatium. All of the Achromatium species identified in a freshwater sediment from Rydal Water, Cumbria, United Kingdom, which were distinguishable only by FISH, assimilated both [(14)C]bicarbonate and [(14)C]acetate. This extends previous findings that Achromatium spp. present at another location could only utilize organic carbon sources. Achromatium spp., therefore, probably exhibit a range of physiologies, i.e., facultative chemolithoautotrophy, mixotrophy, and chemoorganoheterotrophy, similar to other large sulfur bacteria (e.g., Beggiatoa spp.).
摘要
联合显微放射自显影术和荧光原位杂交技术（FISH）被用于研究无色硫细菌属未培养细菌中的碳代谢。在英国坎布里亚郡瑞德尔湖的淡水沉积物中鉴定出的所有无色硫细菌物种，仅通过FISH才能区分，它们同时吸收了[¹⁴C]碳酸氢盐和[¹⁴C]乙酸盐。这扩展了之前的研究结果，即存在于另一个地点的无色硫细菌只能利用有机碳源。因此，无色硫细菌可能表现出一系列生理特性，即兼性化能无机自养、混合营养和化能有机异养，类似于其他大型硫细菌（如贝氏硫菌属）。
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