大肠杆菌中的诱导性易错修复
Inducible error-prone repair in Escherichia coli.
作者信息
Sedgwick S G
出版信息
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2753-7. doi: 10.1073/pnas.72.7.2753.
Abstract
A hypothesis that ultraviolet-induced mutagenesis arises from the induction of an error-prone mode of postreplication repair that requires the exrA+ recA+ genotype has been tested with alkaline sucrose gradient centrifugation coupled with assays of fixation determined by loss of photoreversibility. The inhibitor of protein synthesis, chloramphenicol, added before irradiation, prevented a small amount of postreplication repair and completely eliminated mutation fixation in E. coli WP2s uvrA. However, chloramphenicol did not affect strand joining: (a) in uvrA bacteria allowed 20 min of growth between irradiation and antibiotic treatment; (b) in nonmutable uvrA exrA bacteria; and (c) in uvrA tif bacteria grown at 42 degrees for 70 min before irradiation. These observations indicate that an inducible product is involved in a fraction of postreplication repair and is responsible for induced mutagenesis.
摘要
一种假说认为，紫外线诱导的诱变源于诱导一种易错的复制后修复模式，这种模式需要exrA+ recA+基因型，该假说已通过碱性蔗糖梯度离心结合光可逆性丧失所确定的固定测定进行了检验。在照射前添加蛋白质合成抑制剂氯霉素，可阻止少量复制后修复，并完全消除大肠杆菌WP2s uvrA中的突变固定。然而，氯霉素并不影响链连接：(a) 在uvrA细菌中，在照射和抗生素处理之间允许生长20分钟；(b) 在不可突变的uvrA exrA细菌中；以及(c) 在uvrA tif细菌中，在照射前于42摄氏度生长70分钟。这些观察结果表明，一种可诱导产物参与了一部分复制后修复，并负责诱导诱变。
相似文献
1
Inducible error-prone repair in Escherichia coli.大肠杆菌中的诱导性易错修复
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2753-7. doi: 10.1073/pnas.72.7.2753.
2
Genetic and kinetic evidence for different types of postreplication repair in Escherichia coli B.大肠杆菌B中不同类型复制后修复的遗传学和动力学证据。
J Bacteriol. 1975 Jul;123(1):154-61. doi: 10.1128/jb.123.1.154-161.1975.
3
4
Postreplication repair gap filling in an Escherichia coli strain deficient in dnaB gene product.在缺乏dnaB基因产物的大肠杆菌菌株中进行复制后修复间隙填补。
Basic Life Sci. 1975;5A:325-9. doi: 10.1007/978-1-4684-2895-7_44.
5
Effects of chloramphenicol and caffeine on postreplication repair in uvr A- umuC- und uvrA- recF- strains of Escherichia coli K-12.氯霉素和咖啡因对大肠杆菌K-12的uvrA-umuC-及uvrA-recF-菌株中复制后修复的影响。
Mol Gen Genet. 1977 Nov 14;156(2):115-20. doi: 10.1007/BF00283483.
6
7
Role of DNA polymerase I in postreplication repair: a reexamination with Escherichia coli delta polA.DNA聚合酶I在复制后修复中的作用：用大肠杆菌δpolA进行的重新研究
J Bacteriol. 1987 Oct;169(10):4559-64. doi: 10.1128/jb.169.10.4559-4564.1987.
8
Repair of DNA damage produced by gamma-radiation in Escherichia coli K-12 and a radiation-sensitive exrA derivative during inhibition of protein synthesis and normal DNA replication by chloramphenicol.氯霉素抑制蛋白质合成和正常DNA复制期间，大肠杆菌K-12及其辐射敏感的exrA衍生物中γ辐射产生的DNA损伤修复。
J Gen Microbiol. 1973 Jul;77(1):99-108. doi: 10.1099/00221287-77-1-99.
9
Requirement for protein synthesis in rec-dependent repair of deoxyribonucleic acid in Escherichia coli after ultraviolet or X irradiation.紫外线或X射线照射后大肠杆菌中依赖rec的脱氧核糖核酸修复过程中蛋白质合成的需求
J Bacteriol. 1972 Aug;111(2):575-85. doi: 10.1128/jb.111.2.575-585.1972.
10
Persistence and decay of thermoinducible error-prone repair activity in nonfilamentous derivatives of tif-1, Escherichia coli B/r: the timing of some critical events in ultraviolet mutagenesis.大肠杆菌B/r的tif-1非丝状衍生物中热诱导易错修复活性的持久性和衰减：紫外线诱变中一些关键事件的时间安排
Mol Gen Genet. 1975 Dec 29;142(2):87-103. doi: 10.1007/BF00266092.
引用本文的文献
1
A matter of life or death: modeling DNA damage and repair in bacteria.生死攸关：在细菌中模拟 DNA 损伤与修复。
Biophys J. 2011 Feb 16;100(4):814-21. doi: 10.1016/j.bpj.2010.12.3713.
2
Deoxyribonucleic Acid Repair in Bacillus subtilis: Development of Competent Cells into a Tester for Carcinogens.枯草芽孢杆菌中的脱氧核糖核酸修复：将感受态细胞开发成致癌物检测工具。
Appl Environ Microbiol. 1980 Apr;39(4):854-8. doi: 10.1128/aem.39.4.854-858.1980.
3
Repair and mutagenesis of plasmid DNA modified by ultraviolet irradiation or N-acetoxy-N-2-acetylaminofluorene.紫外线照射或N-乙酰氧基-N-2-乙酰氨基芴修饰的质粒DNA的修复与诱变
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4133-7. doi: 10.1073/pnas.79.13.4133.
4
Characterization of postreplication repair in Saccharomyces cerevisiae and effects of rad6, rad18, rev3 and rad52 mutations.酿酒酵母中复制后修复的特征以及rad6、rad18、rev3和rad52基因突变的影响。
Mol Gen Genet. 1981;184(3):471-8. doi: 10.1007/BF00352525.
5
UV-induced repair in Escherichia coli B/r Hcr-thy trp cells: its dependence on UV damage.大肠杆菌B/r Hcr-thy trp细胞中的紫外线诱导修复：其对紫外线损伤的依赖性。
Folia Microbiol (Praha). 1982;27(1):65-8. doi: 10.1007/BF02883841.
6
Repair of DNA double-strand breaks in Escherichia coli cells requires synthesis of proteins that can be induced by UV light.大肠杆菌细胞中DNA双链断裂的修复需要合成可被紫外线诱导的蛋白质。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3450-3. doi: 10.1073/pnas.78.6.3450.
7
Involvement of genes uvrD and recB in separate mutagenic deoxyribonucleic acid repair pathways in Escherichia coli K-12 uvrB5 and B/r uvrA155.uvrD和recB基因参与大肠杆菌K-12 uvrB5和B/r uvrA155中不同的诱变脱氧核糖核酸修复途径。
J Bacteriol. 1980 Jul;143(1):212-20. doi: 10.1128/jb.143.1.212-220.1980.
8
Inducible sfi dependent division inhibition in Escherichia coli.大肠杆菌中可诱导的sfi依赖性分裂抑制
Mol Gen Genet. 1980;177(4):629-36. doi: 10.1007/BF00272673.
9
Inducible repair system in Haemophilus influenzae unaccompanied by mutation.流感嗜血杆菌中无突变伴随的可诱导修复系统。
J Bacteriol. 1980 Jul;143(1):516-9. doi: 10.1128/jb.143.1.516-519.1980.
10
Regulation of the Escherichia coli K-12 uvrB operon.大肠杆菌K-12 uvrB操纵子的调控
J Bacteriol. 1982 May;150(2):676-85. doi: 10.1128/jb.150.2.676-685.1982.
本文引用的文献
1
UV-reactivation of bacteriophage lambda in excision repair-deficient hosts: independence of red functions and attachment regions.噬菌体λ在切除修复缺陷宿主中的紫外线复活：red功能和附着区域的独立性
Virology. 1971 Feb;43(2):504-6. doi: 10.1016/0042-6822(71)90322-9.
2
Ultraviolet reactivation and ultraviolet mutagenesis of lambda in different genetic systems.λ噬菌体在不同遗传系统中的紫外线复活和紫外线诱变
Virology. 1971 Feb;43(2):495-503. doi: 10.1016/0042-6822(71)90321-7.
3
Radiation-sensitive mutants of T4D. I. T4y: a new radiation-sensitive mutant; effect of the mutation on radiation survival, growth and recombination.T4D的辐射敏感突变体。I. T4y：一种新的辐射敏感突变体；突变对辐射存活、生长和重组的影响。
Mutat Res. 1969 Nov-Dec;8(3):431-9. doi: 10.1016/0027-5107(69)90060-8.
4
The radiation sensitivity of Escherichia coli B: a hypothesis relating filament formation and prophage induction.大肠杆菌B的辐射敏感性：关于丝状形成和原噬菌体诱导的一种假说。
Proc Natl Acad Sci U S A. 1967 May;57(5):1275-9. doi: 10.1073/pnas.57.5.1275.
5
The range of action of genes controlling radiation sensitivity in Escherichia coli.控制大肠杆菌辐射敏感性的基因作用范围。
Mutat Res. 1965 Apr;2(2):111-31. doi: 10.1016/0027-5107(65)90042-4.
6
Ultraviolet-sensitive targets in the enzyme-synthesizing apparatus of Escherichia coli.大肠杆菌酶合成装置中的紫外线敏感靶点。
J Bacteriol. 1967 Apr;93(4):1210-9. doi: 10.1128/jb.93.4.1210-1219.1967.
7
Transduction studies on the role of a rec+ gene in the ultraviolet induction of prophage lambda.关于rec+基因在紫外线诱导噬菌体λ原噬菌体过程中作用的转导研究。
J Mol Biol. 1967 Jan 28;23(2):117-33. doi: 10.1016/s0022-2836(67)80021-4.
8
A note on the mechanism of UV mutagenesis in Escherichia coli.关于大肠杆菌紫外线诱变机制的一篇笔记。
Mutat Res. 1966 Aug;3(4):273-9. doi: 10.1016/0027-5107(66)90034-0.
9
Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA.大肠杆菌K-12中控制从DNA切除嘧啶二聚体和某些其他诱变产物的三个基因座。
Genetics. 1966 Jun;53(6):1119-36. doi: 10.1093/genetics/53.6.1119.
10
Ultraviolet-induced lethality and reversion to prototrophy in Escherichia coli strains with normal and reduced dark repair ability.紫外线诱导具有正常和降低的暗修复能力的大肠杆菌菌株的致死率及回复为原养型。
Photochem Photobiol. 1965 Jun;4(3):563-8. doi: 10.1111/j.1751-1097.1965.tb09774.x.
Zotero 插件