大肠杆菌中的诱导性易错修复
Inducible error-prone repair in Escherichia coli.
作者信息
Sedgwick S G
出版信息
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2753-7. doi: 10.1073/pnas.72.7.2753.
Abstract
A hypothesis that ultraviolet-induced mutagenesis arises from the induction of an error-prone mode of postreplication repair that requires the exrA+ recA+ genotype has been tested with alkaline sucrose gradient centrifugation coupled with assays of fixation determined by loss of photoreversibility. The inhibitor of protein synthesis, chloramphenicol, added before irradiation, prevented a small amount of postreplication repair and completely eliminated mutation fixation in E. coli WP2s uvrA. However, chloramphenicol did not affect strand joining: (a) in uvrA bacteria allowed 20 min of growth between irradiation and antibiotic treatment; (b) in nonmutable uvrA exrA bacteria; and (c) in uvrA tif bacteria grown at 42 degrees for 70 min before irradiation. These observations indicate that an inducible product is involved in a fraction of postreplication repair and is responsible for induced mutagenesis.
摘要
一种假说认为，紫外线诱导的诱变源于诱导一种易错的复制后修复模式，这种模式需要exrA+ recA+基因型，该假说已通过碱性蔗糖梯度离心结合光可逆性丧失所确定的固定测定进行了检验。在照射前添加蛋白质合成抑制剂氯霉素，可阻止少量复制后修复，并完全消除大肠杆菌WP2s uvrA中的突变固定。然而，氯霉素并不影响链连接：(a) 在uvrA细菌中，在照射和抗生素处理之间允许生长20分钟；(b) 在不可突变的uvrA exrA细菌中；以及(c) 在uvrA tif细菌中，在照射前于42摄氏度生长70分钟。这些观察结果表明，一种可诱导产物参与了一部分复制后修复，并负责诱导诱变。
相似文献
1
Inducible error-prone repair in Escherichia coli.
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2753-7. doi: 10.1073/pnas.72.7.2753.
2
Genetic and kinetic evidence for different types of postreplication repair in Escherichia coli B.
J Bacteriol. 1975 Jul;123(1):154-61. doi: 10.1128/jb.123.1.154-161.1975.
3
[Postreplication DNA repair in Escherichia coli cells. II. The necessity of primase for constitutive repair].
Tsitologiia. 1983 Jun;25(6):703-6.
4
Postreplication repair gap filling in an Escherichia coli strain deficient in dnaB gene product.
Basic Life Sci. 1975;5A:325-9. doi: 10.1007/978-1-4684-2895-7_44.
5
Effects of chloramphenicol and caffeine on postreplication repair in uvr A- umuC- und uvrA- recF- strains of Escherichia coli K-12.
Mol Gen Genet. 1977 Nov 14;156(2):115-20. doi: 10.1007/BF00283483.
6
[Radioadaptive enhancement of the repair of UV-induced postreplication gaps in Escherichia coli cells deficient in DNA repair].
Tsitologiia. 2002;44(5):499-501.
7
Role of DNA polymerase I in postreplication repair: a reexamination with Escherichia coli delta polA.
J Bacteriol. 1987 Oct;169(10):4559-64. doi: 10.1128/jb.169.10.4559-4564.1987.
8
Repair of DNA damage produced by gamma-radiation in Escherichia coli K-12 and a radiation-sensitive exrA derivative during inhibition of protein synthesis and normal DNA replication by chloramphenicol.
J Gen Microbiol. 1973 Jul;77(1):99-108. doi: 10.1099/00221287-77-1-99.
9
Requirement for protein synthesis in rec-dependent repair of deoxyribonucleic acid in Escherichia coli after ultraviolet or X irradiation.
J Bacteriol. 1972 Aug;111(2):575-85. doi: 10.1128/jb.111.2.575-585.1972.
10
Persistence and decay of thermoinducible error-prone repair activity in nonfilamentous derivatives of tif-1, Escherichia coli B/r: the timing of some critical events in ultraviolet mutagenesis.
Mol Gen Genet. 1975 Dec 29;142(2):87-103. doi: 10.1007/BF00266092.
引用本文的文献
1
A matter of life or death: modeling DNA damage and repair in bacteria.
Biophys J. 2011 Feb 16;100(4):814-21. doi: 10.1016/j.bpj.2010.12.3713.
2
Deoxyribonucleic Acid Repair in Bacillus subtilis: Development of Competent Cells into a Tester for Carcinogens.
Appl Environ Microbiol. 1980 Apr;39(4):854-8. doi: 10.1128/aem.39.4.854-858.1980.
3
Repair and mutagenesis of plasmid DNA modified by ultraviolet irradiation or N-acetoxy-N-2-acetylaminofluorene.
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4133-7. doi: 10.1073/pnas.79.13.4133.
4
Characterization of postreplication repair in Saccharomyces cerevisiae and effects of rad6, rad18, rev3 and rad52 mutations.
Mol Gen Genet. 1981;184(3):471-8. doi: 10.1007/BF00352525.
5
UV-induced repair in Escherichia coli B/r Hcr-thy trp cells: its dependence on UV damage.
Folia Microbiol (Praha). 1982;27(1):65-8. doi: 10.1007/BF02883841.
6
Repair of DNA double-strand breaks in Escherichia coli cells requires synthesis of proteins that can be induced by UV light.
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3450-3. doi: 10.1073/pnas.78.6.3450.
7
Involvement of genes uvrD and recB in separate mutagenic deoxyribonucleic acid repair pathways in Escherichia coli K-12 uvrB5 and B/r uvrA155.
J Bacteriol. 1980 Jul;143(1):212-20. doi: 10.1128/jb.143.1.212-220.1980.
8
Inducible sfi dependent division inhibition in Escherichia coli.
Mol Gen Genet. 1980;177(4):629-36. doi: 10.1007/BF00272673.
9
Inducible repair system in Haemophilus influenzae unaccompanied by mutation.
J Bacteriol. 1980 Jul;143(1):516-9. doi: 10.1128/jb.143.1.516-519.1980.
10
Regulation of the Escherichia coli K-12 uvrB operon.
J Bacteriol. 1982 May;150(2):676-85. doi: 10.1128/jb.150.2.676-685.1982.
本文引用的文献
1
UV-reactivation of bacteriophage lambda in excision repair-deficient hosts: independence of red functions and attachment regions.
Virology. 1971 Feb;43(2):504-6. doi: 10.1016/0042-6822(71)90322-9.
2
Ultraviolet reactivation and ultraviolet mutagenesis of lambda in different genetic systems.
Virology. 1971 Feb;43(2):495-503. doi: 10.1016/0042-6822(71)90321-7.
3
Radiation-sensitive mutants of T4D. I. T4y: a new radiation-sensitive mutant; effect of the mutation on radiation survival, growth and recombination.
Mutat Res. 1969 Nov-Dec;8(3):431-9. doi: 10.1016/0027-5107(69)90060-8.
4
The radiation sensitivity of Escherichia coli B: a hypothesis relating filament formation and prophage induction.
Proc Natl Acad Sci U S A. 1967 May;57(5):1275-9. doi: 10.1073/pnas.57.5.1275.
5
The range of action of genes controlling radiation sensitivity in Escherichia coli.
Mutat Res. 1965 Apr;2(2):111-31. doi: 10.1016/0027-5107(65)90042-4.
6
Ultraviolet-sensitive targets in the enzyme-synthesizing apparatus of Escherichia coli.
J Bacteriol. 1967 Apr;93(4):1210-9. doi: 10.1128/jb.93.4.1210-1219.1967.
7
Transduction studies on the role of a rec+ gene in the ultraviolet induction of prophage lambda.
J Mol Biol. 1967 Jan 28;23(2):117-33. doi: 10.1016/s0022-2836(67)80021-4.
8
A note on the mechanism of UV mutagenesis in Escherichia coli.
Mutat Res. 1966 Aug;3(4):273-9. doi: 10.1016/0027-5107(66)90034-0.
9
Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA.
Genetics. 1966 Jun;53(6):1119-36. doi: 10.1093/genetics/53.6.1119.
10
Ultraviolet-induced lethality and reversion to prototrophy in Escherichia coli strains with normal and reduced dark repair ability.
Photochem Photobiol. 1965 Jun;4(3):563-8. doi: 10.1111/j.1751-1097.1965.tb09774.x.
Zotero 插件