de Oliveira Milene Castilhos, Krueger Gabriel Francisco, Sganzerla Juliana Tomaz, Gassen Humberto Thomazi, Hernández Pedro Antonio González, Allgayer Mariangela da Costa, Miguens-Jr Sergio Augusto Quevedo
Department of Oral Medicine, Graduate Program in Dentistry, Universidade Luterana do Brasil, Canoas, RS, Brazil.
Department of Oral Surgery, Graduate Program in Dentistry, Universidade Luterana do Brasil, Canoas, RS, Brazil.
J Lasers Med Sci. 2021 Aug 19;12:e45. doi: 10.34172/jlms.2021.45. eCollection 2021.
This study aimed to investigate the effect of low-level laser therapy (LLLT) on the blood cell count when applied to parotid glands of rats irradiated by volumetric modular arc therapy (VMAT). Thirty-two adult male Wistar rats were used in this study. Samples were randomly assigned to three groups: control group (CG, n = 8), immediate laser group (24 hours) (ILG, n=12), and late laser group (120 hours) (LLG, n=12). The two laser groups were previously subjected to VMAT radiotherapy in a single dose of 12 Gy. LLLT with an AsGaAl laser (660 nm, 100 mW) was applied at three points in the region of the parotid glands, right side, with the energy of 2 J per point (20s, 70 J/cm) and a spot size of 0.0028 cm for 10 consecutive days. In the euthanasia, blood samples were obtained by cardiac puncture. The samples from each group were processed by an automatic method and analyzed for erythrogram, leukogram and platelet count values. The data were analyzed by ANOVA and each LLLT time point was analyzed in relation to the control group, with a significance level less than 0.05. Groups using LLLT had higher red blood cell counts, being higher in the LLG ( = 0.000). The hematimetric indices MCV ( = 0.002) and MCH ( = 0.009) were lower than the control group, especially when compared to the group using LLLT 120h after radiotherapy (LLG). White blood cell counts were lower in the groups with radiotherapy and immediate use of LLLT (ILG) ( = 0.011), mainly at the expense of lymphocytes ( = 0.002). The results suggest a potential systemic effect of LLLT, especially on circulating red blood cell counts, regardless of their time of immediate or late use of radiotherapy.
本研究旨在探讨低强度激光疗法（LLLT）应用于容积调强弧形放疗（VMAT）照射的大鼠腮腺时对血细胞计数的影响。本研究使用了32只成年雄性Wistar大鼠。样本被随机分为三组：对照组（CG，n = 8）、即刻激光组（24小时）（ILG，n = 12）和延迟激光组（120小时）（LLG，n = 12）。两个激光组先前接受了单次剂量为12 Gy的VMAT放射治疗。使用砷镓铝激光（660 nm，100 mW）在右侧腮腺区域的三个点进行LLLT，每点能量为2 J（20秒，70 J/cm），光斑尺寸为0.0028 cm，连续照射10天。在安乐死时，通过心脏穿刺采集血样。每组样本采用自动方法进行处理，并分析红细胞计数、白细胞计数和血小板计数值。数据采用方差分析进行分析，每个LLLT时间点与对照组进行比较分析，显著性水平小于0.05。使用LLLT的组红细胞计数较高，在LLG中更高（P = 0.000）。血细胞比容指数MCV（P = 0.002）和MCH（P = 0.009）低于对照组，尤其是与放疗后120小时使用LLLT的组（LLG）相比。放疗并即刻使用LLLT的组（ILG）白细胞计数较低（P = 0.011），主要是淋巴细胞减少（P = 0.002）。结果表明LLLT具有潜在的全身效应，尤其是对循环红细胞计数，无论放疗后即刻或延迟使用LLLT。
