Hostnik P
Institute of Microbiology, Veterinary Faculty, Ljubljana, Slovenia.
J Vet Med B Infect Dis Vet Public Health. 2000 Aug;47(6):423-7. doi: 10.1046/j.1439-0450.2000.00362.x.
The fluorescent antibody virus neutralization test (FAVN) for the detection of antibodies against rabies virus was modified by using a monoclonal anti-rabies antibodies and peroxidase anti-mouse conjugate instead of a fluorescent anti-rabies conjugate. The results were read on an automatic multi-channel spectrophotometer. A total of 182 serum samples from dogs were tested by both the original and modified FAVN methods and the results were compared. Good correlation was found between the two tests. Practically, the modified FAVN test was quicker and could be used for a larger number of samples.
用于检测狂犬病病毒抗体的荧光抗体病毒中和试验(FAVN)进行了改进,使用单克隆抗狂犬病抗体和过氧化物酶抗小鼠共轭物替代荧光抗狂犬病共轭物。结果在自动多通道分光光度计上读取。用原始和改进的FAVN方法对总共182份犬血清样本进行了检测,并对结果进行了比较。两种检测方法之间发现有良好的相关性。实际上,改进后的FAVN试验更快,可用于检测更多样本。