Ondrejková A, Süli J, Ondrejka R, Slepecká E, Prokeš M, Čechvala P, Supuka P
Pol J Vet Sci. 2015;18(1):47-51. doi: 10.1515/pjvs-2015-0006.
In the presented work, we compared the results of determination of rabies antibodies using three in vitro methods: rapid fluorescent focus inhibition test (RFFIT), fluorescent antibody virus neutralisation test (FAVNT) and the immunoenzymatic assay (ELISA). 196 dog sera samples were examined with FAVNT, RFFIT methods and the ELISA test. Sera with low and sufficiently high titre of antibodies had a similar result in determining by all methods. A critical level of rabies antibodies close to the required protection level (0.5 IU/cm3) was seen in sera of 18 dogs (9.18%); these were the sera obtained after primary vaccination of dogs. At this level, even small differences can cause a change in the assessment of the patient's serum seronegativity or seropositivity. Therefore, it is important to choose the appropriate method that has sufficiently strict criteria while having a good reproducibility.
在本研究中，我们比较了三种体外方法检测狂犬病抗体的结果：快速荧光灶抑制试验（RFFIT）、荧光抗体病毒中和试验（FAVNT）和免疫酶测定法（ELISA）。采用FAVNT、RFFIT方法和ELISA试验检测了196份犬血清样本。抗体滴度低和足够高的血清在所有方法检测中结果相似。18只犬（9.18%）的血清中狂犬病抗体临界水平接近所需保护水平（0.5 IU/cm³）；这些血清是犬初次接种疫苗后获得的。在此水平下，即使是微小差异也可能导致患者血清血清阴性或血清阳性评估的改变。因此，选择具有足够严格标准且重现性良好的合适方法很重要。
