Kolberg J, Nordhagen R
Transfusion. 1975 Jul-Aug;15(4):334-9. doi: 10.1046/j.1537-2995.1975.15476034553.x.
Some factors affecting the sensitivity of the automated methods for blood group antibody detection have been evaluated. The experiments revealed influencing differences between various albumin preparations. In the BMC method, one lot of albumin permitted no significant antibody detection. In the LISP technique, a plateau of maximum Polybrene activity was found. The beginning of this plateau depended on both the albumin preparation and the Polybrene lot. In the BMC method, methyl cellulose gave optimal sensitivity within a concentration range of 0.3 to 0.5 per cent. The stability of test cells stored in ACD at 4 C was studied. All test cells could be used safely up to two weeks. Cells from different donors showed variable reactivity after three weeks.
已对一些影响血型抗体检测自动化方法灵敏度的因素进行了评估。实验揭示了各种白蛋白制剂之间的影响差异。在BMC方法中，一批白蛋白无法检测到显著抗体。在LISP技术中，发现了聚凝胺活性的最大值平台。该平台的起始点取决于白蛋白制剂和聚凝胺批次。在BMC方法中，甲基纤维素在0.3%至0.5%的浓度范围内具有最佳灵敏度。研究了储存在4℃的ACD中的测试细胞的稳定性。所有测试细胞在两周内均可安全使用。三周后，来自不同供体的细胞显示出不同的反应性。
