Souza M B L D, Rácz M L, Leite J P G, Soares C M A, Martins R M B, Munford V, Cardoso D D P
Laboratório de Virologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua Delenda Rezende de Melo Esquina Com 1a Avenida s/n, Setor Universitário, 74605-050 Goiânia, Goiás, Brazil.
Eur J Clin Microbiol Infect Dis. 2003 Jul;22(7):441-3. doi: 10.1007/s10096-003-0952-0. Epub 2003 Jun 27.
Fecal samples positive for rotavirus group A ( n=120) were analyzed by enzyme immunoassay-monoclonal antibody (EIA-MAb) serotyping and/or reverse transcription/multiplex polymerase chain reaction (PCR) amplification to determine the prevalence of the [P] and G genotypes. The most prevalent G genotype/serotype detected was G1 (76.7%), followed by G2 (5.0%). Six samples were characterized as G9 by multiplex PCR, and one sample was characterized as G3 by EIA-MAb. The combinations of [P] and G genotypes found were P[8] and G1 (20.8%), P[6] and G1 (10.8%), P[6] and G9 (4.2%), P[8] and G2 (1.7%), and P[6] and G2 (0.8%). The diversity of rotavirus group A [P] and G genotypes/serotypes reinforces the need for continuous characterization of rotaviruses circulating in populations in Brazil.
对120份A组轮状病毒检测呈阳性的粪便样本进行酶免疫测定-单克隆抗体（EIA-MAb）血清分型分析和/或逆转录/多重聚合酶链反应（PCR）扩增，以确定[P]和G基因型的流行情况。检测到的最常见G基因型/血清型为G1（76.7%），其次是G2（5.0%）。6份样本通过多重PCR鉴定为G9，1份样本通过EIA-MAb鉴定为G3。发现的[P]和G基因型组合为P[8]和G1（20.8%）、P[6]和G1（10.8%）、P[6]和G9（4.2%）、P[8]和G2（1.7%）以及P[6]和G2（0.8%）。A组轮状病毒[P]和G基因型/血清型的多样性凸显了持续鉴定巴西人群中传播的轮状病毒的必要性。
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