Coluchi Norma, Munford Veridiana, Manzur Julio, Vazquez Cynthia, Escobar Mabel, Weber Ernesto, Mármol Perla, Rácz Maria Lucia
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374 São Paulo, SP, 05508-900 Brazil.
J Clin Microbiol. 2002 May;40(5):1709-14. doi: 10.1128/JCM.40.5.1709-1714.2002.
Of a total of 220 stool specimens from children with acute diarrhea, mostly under the age of 3 years, collected in Paraguay between January 1999 and March 2000, 70 (31.8%) were found positive for rotaviruses (RV). Positive samples were characterized by electropherotyping and subgrouping. Sixty-one (87.1%) were classified as group A, subgroup II; one (1.4%) was classified as group A, subgroup I; six (8.6%) were group A, non-I non-II; and two (2.9%) were not tested. RV strains were G and P genotyped by reverse transcription-PCR. The following G types were detected: G4 (34.3%), G1 (21.4%), G2 (1.4%), and G9 (5.7%). Mixtures of human and animal genotypes were detected in 15 (21.4%) samples, and 11 samples (15.7%) were nontypeable. The following P types were detected: P[8] (48.6%), P[4] (1.4%), and P[1] (1.4%). A mixed type was found in 10% of samples, and an unexpectedly high percentage (38.6%) of nontypeable samples was found. The common human G- and P-type combinations P[8], G4 (15.7%) and P[8], G1 (14.2%) were detected. Mixed human and animal genotypes were observed as the following combinations: G4 + G5, G4 + G5 + G10, and G1 + G10 for G types and P[8]-P[1] for P types. The emerging G9 genotype was detected in four samples. These results show for the first time the diversity of RV circulating among children in Paraguay and contribute to the knowledge of this pathogen required to devise strategies to prevent diarrheal illness in this country. The finding of mixed genotypes may indicate interspecies transmission of RV between humans and animals.
1999年1月至2000年3月期间在巴拉圭收集了220份急性腹泻儿童的粪便样本，这些儿童大多年龄在3岁以下，其中70份（31.8%）轮状病毒（RV）检测呈阳性。对阳性样本进行了电泳分型和亚组分析。61份（87.1%）被归类为A组，II亚组；1份（1.4%）被归类为A组，I亚组；6份（8.6%）为A组，非I非II；2份（2.9%）未进行检测。通过逆转录聚合酶链反应对RV毒株进行G和P基因分型。检测到以下G型：G4（34.3%）、G1（21.4%）、G2（1.4%）和G9（5.7%）。15份（21.4%）样本检测到人和动物基因型的混合，11份样本（15.7%）无法分型。检测到以下P型：P[8]（48.6%）、P[4]（1.4%）和P[1]（1.4%）。10%的样本发现为混合型，无法分型的样本比例意外较高（38.6%）。检测到常见的人G型和P型组合P[8]，G4（15.7%）和P[8]，G1（14.2%）。观察到人和动物基因型的混合如下组合：G型为G4 + G5、G4 + G5 + G10和G1 + G10，P型为P[8]-P[1]。在4份样本中检测到新出现的G9基因型。这些结果首次显示了巴拉圭儿童中循环的RV的多样性，并有助于了解该病原体，以制定该国预防腹泻病的策略。混合基因型的发现可能表明RV在人和动物之间的种间传播。
