Schmidt-Gollwitzer M, Saxena B B
Acta Endocrinol (Copenh). 1975 Oct;80(2):262-74. doi: 10.1530/acta.0.0800262.
A simple and rapid homologous radioimmunoassay of human prolactin (hPRL), by the use of 66% ethanol containing 6.6% ammonium acetate for the separation of free and bound hormones, has been established. PRL levels showed marked individual and daily variations as well as a circadian periodicity in both men and women. Levels of plasma PRL rose with puberty and decreased during post-menopause and in elderly men (ages 60-73 years). There was no marked change in plasma PRL levels at midcycle; however, in some subjects, luteal levels were slightly higher than follicular levels. Administration of oestradiol-17beta caused marked increase in plasma PRL levels during menstrual cycle. During pregnancy, the plasma PRL levels rose as high as 200 ng/ml and decreased within a week or two post-partum. During lactation, the levels were higher and increased with suckling. In a patient with habitual abortion, the plasma PRL level showed a sudden drop prior to abortion. Administration of LH-RH had no effect on the secretion of PRL in human subjects.
通过使用含6.6%醋酸铵的66%乙醇来分离游离和结合激素，建立了一种简单快速的人催乳素（hPRL）同源放射免疫测定法。催乳素水平在男性和女性中均表现出明显的个体差异和每日变化以及昼夜节律。血浆催乳素水平在青春期升高，在绝经后和老年男性（60 - 73岁）中降低。在月经周期中期，血浆催乳素水平没有明显变化；然而，在一些受试者中，黄体期水平略高于卵泡期水平。在月经周期中，给予17β-雌二醇会导致血浆催乳素水平显著升高。在怀孕期间，血浆催乳素水平可高达200 ng/ml，并在产后一两周内下降。在哺乳期，水平较高且随哺乳而增加。在一名习惯性流产患者中，流产前血浆催乳素水平突然下降。给予促黄体生成素释放激素（LH - RH）对人体催乳素的分泌没有影响。
