Schierhölter R, Honegger H
Adv Ophthalmol. 1975;31:34-99.
On a preparation of the corneal endothelium membrane from a rabbit, the morphological structure of the endothelium is examined under normal physiological conditions and during regeneration after abrasion by mechanical means. Thematerial which is submitted here is outlined in two chapters: (1) The morphology of the normal corneal endothelium; (a) in the contest of the literature on the subject, (b) in comparison with different methods of preparation. (2) The morphology of endothelium regeneration; (a) in the literature as physiological regeneration and, after experimental abrasion, as a model for human endothelium pathology, (b)as a systematic examination at different points of time after mechanical abrasion and in single phases of indirect and direct cell multiplication. After a survey of the comprehensive literature on the subject, several histological fixations and methodds of endothelium preparation as a membrane are given. In a comparison of the findings of the presentation of the normal endothelium, these methods are described in virw of a specific presentation of pathological endothelium rocesses. On the basis of the dyeing characteristics of the cells after intraocular pre-fixation; the evidence of the viscous mucopolysaccharoid membrane on the cell surface (the membrane having been dissected from the endothelium) is discussed...
在从兔子制备角膜内皮细胞膜的过程中,在正常生理条件下以及机械磨损后再生过程中检查内皮的形态结构。此处提交的材料分为两章概述:(1)正常角膜内皮的形态;(a)在该主题的文献背景下,(b)与不同制备方法的比较。(2)内皮再生的形态;(a)在文献中作为生理再生,在实验性磨损后作为人类内皮病理学模型,(b)作为机械磨损后不同时间点以及间接和直接细胞增殖的单个阶段的系统检查。在对该主题的综合文献进行综述后,给出了几种组织学固定方法和作为膜的内皮制备方法。在比较正常内皮呈现的结果时,根据病理性内皮过程的特定呈现来描述这些方法。基于眼内预固定后细胞的染色特性,讨论了细胞表面粘性粘多糖膜的证据(该膜已从内皮中分离出来)……