Cradock-Watson J E, Ridehalgh M K, Chantler S
Ann N Y Acad Sci. 1975 Jun 30;254:385-93. doi: 10.1111/j.1749-6632.1975.tb29188.x.
The indirect immunofluorescence technique has been used to study specific IgG, IgA, and IgM antibodies in adults with acute rubella, volunteers who receive attentuated rubella vaccine, and infants with suspected congenital infection. In acute rubella, IgA and IgM antibodies reached peak titers during the second week after the rash and then declined, but specific IgG persisted. A similar pattern of response occurred after rubella vaccination, but titers were lower. Occasionally, fluorescent staining failed to detect specific IgM in whole serum but demonstrated it clearly in sucrose density gradient fractions. The improvement in fluorescence obtained by staining serum fractions was particularly striking in samples obtained from congenitally infected infants. In these cases, IgM staining with whole serum was often poor, but when fractions were tested, specific IgM was demonstrated in nearly all samples obtained within 9 months of birth. IgM antibody was seldom detected after the first year of life, but IgG antibody persisted. IgA antibody was not detected in congenital rubella.
间接免疫荧光技术已被用于研究患急性风疹的成人、接种减毒风疹疫苗的志愿者以及疑似先天性感染的婴儿体内的特异性IgG、IgA和IgM抗体。在急性风疹中，IgA和IgM抗体在出疹后第二周达到滴度峰值，然后下降，但特异性IgG持续存在。接种风疹疫苗后出现类似的反应模式，但滴度较低。偶尔，荧光染色在全血清中未能检测到特异性IgM，但在蔗糖密度梯度分离物中能清楚地显示出来。通过对血清分离物进行染色获得的荧光改善在先天性感染婴儿的样本中尤为显著。在这些病例中，用全血清进行IgM染色往往效果不佳，但对分离物进行检测时，几乎所有在出生后9个月内采集的样本中都能检测到特异性IgM。1岁以后很少检测到IgM抗体，但IgG抗体持续存在。先天性风疹中未检测到IgA抗体。
