Salonen E M, Hovi T, Meurman O, Vesikari T, Vaheri A
J Med Virol. 1985 May;16(1):1-9. doi: 10.1002/jmv.1890160102.
Rubella-specific IgD and IgE antibodies were determined with a solid-phase enzyme immunoassay using enzyme-labeled heavy-chain specific anti-immuno-globulins, and the antibody responses in rubella infection were compared to IgM, IgA, and IgG antibodies. IgD and IgE antibodies increased rapidly after the onset of infection, remained at a high level for at least 2 months, and declined slightly by 6 months. In comparison, the IgM antibodies decreased more rapidly, whereas the IgG antibodies persisted longer at a steady level. By 6 months the mean levels of the different antibodies had declined from their maximal mean levels as follows: IgM, 52%; IgA, 42%; IgE, 35%; IgD, 29%; and IgG, 8%. Thus IgD and IgE antibodies, in spite of their known short half lives, persisted longer than IgM and IgA antibodies, which limits their diagnostic value. The IgA antibody responses were found too variable to substitute for IgM antibody determination in diagnosis of a recent rubella virus infection from a single serum specimen. Comparison of maternal and cord blood sera indicated that, in addition to IgG antibodies, rubella-specific IgD antibodies were found to cross the placenta.
采用酶标记的重链特异性抗免疫球蛋白的固相酶免疫测定法检测风疹特异性IgD和IgE抗体，并将风疹感染中的抗体反应与IgM、IgA和IgG抗体进行比较。感染开始后，IgD和IgE抗体迅速增加，至少2个月保持在高水平，6个月时略有下降。相比之下，IgM抗体下降更快，而IgG抗体在稳定水平持续更长时间。到6个月时，不同抗体的平均水平从其最高平均水平下降如下：IgM为52%；IgA为42%；IgE为35%；IgD为29%；IgG为8%。因此，尽管已知IgD和IgE抗体的半衰期较短，但它们的持续时间比IgM和IgA抗体更长，这限制了它们的诊断价值。发现IgA抗体反应变化太大，无法从单一血清标本替代IgM抗体测定来诊断近期风疹病毒感染。母体和脐带血血清的比较表明，除了IgG抗体外，还发现风疹特异性IgD抗体可穿过胎盘。
