Gillemaut P, Weil J H
Biochim Biophys Acta. 1975 Oct 1;407(2):240-8. doi: 10.1016/0005-2787(75)90288-9.
Met-tRNA synthetase from Paseolus vulgaris cytoplasm could be separated from its chloroplastic or mitochondrial counterpart by DEAE-cellulose chromatography, but the Met-tRNA synthetase from the two latter organelles could not be distinguished using DEAE-cellulose, hydroxyapatite or CM-Sephadex chromatography. As revealed by reverse-phase chromatography, bean cytoplasm contains 2 tRNAsMet; only one is charged by chloroplast, mitochondrial or Escherichia coli Met-tRNA synthetase. Mitochondria contain, in addition to the 2 cytoplasmic tRNAsMet, 3 mitochondria-spedific tRNAsMet; 2 can be formylated by the mitochondrial or the E. coli transformylase; all 3 are charged by mitochondrial, chloroplastic or E, coli Met-tRNA synthetase; none is charged by the cytoplasmic enzyme. Chloroplasts contain, in addition to the 2 cytoplasmic tRNAsMet, 3 chloroplast-specific tRNAsMet, different from the mitochondrial tRNAsMet; one is formylatable by the chloroplastic or the E. coli transformylase; all 3 are charged by chloroplastic, mitochondrial or E. coli Met-tRNA synthetase; only one is charged by the cytoplasmic enzyme. Of the 3 E. coli tRNAsMet, only the formylatable species can be charged by bean cytoplasmic, chloroplastic or mitochondrial Met-tRNA synthetase.
菜豆细胞质中的甲硫氨酰 - tRNA合成酶可通过DEAE - 纤维素色谱法与叶绿体或线粒体中的对应酶分离，但后两种细胞器中的甲硫氨酰 - tRNA合成酶无法用DEAE - 纤维素、羟基磷灰石或CM - 葡聚糖凝胶色谱法区分。反相色谱显示，菜豆细胞质含有2种甲硫氨酸tRNA；只有一种能被叶绿体、线粒体或大肠杆菌的甲硫氨酰 - tRNA合成酶充电。线粒体除了含有2种细胞质甲硫氨酸tRNA外，还含有3种线粒体特异性甲硫氨酸tRNA；其中2种可被线粒体或大肠杆菌转甲酰酶甲酰化；所有3种都能被线粒体、叶绿体或大肠杆菌的甲硫氨酰 - tRNA合成酶充电；没有一种能被细胞质酶充电。叶绿体除了含有2种细胞质甲硫氨酸tRNA外，还含有3种叶绿体特异性甲硫氨酸tRNA，与线粒体甲硫氨酸tRNA不同；其中一种可被叶绿体或大肠杆菌转甲酰酶甲酰化；所有3种都能被叶绿体、线粒体或大肠杆菌的甲硫氨酰 - tRNA合成酶充电；只有一种能被细胞质酶充电。在3种大肠杆菌甲硫氨酸tRNA中，只有可被甲酰化的那种能被菜豆细胞质、叶绿体或线粒体的甲硫氨酰 - tRNA合成酶充电。
