Bonen L, Gray M W
Nucleic Acids Res. 1980 Jan 25;8(2):319-35. doi: 10.1093/nar/8.2.319.
We show here that mitochondrial-specific ribosomal and transfer RNAs of wheat (Triticum vulgare Vill. [Triticum aestivum L.] var. Thatcher) are encoded by the mitochondrial DNA (mtDNA). Individual wheat mitochondrial rRNA species (26S, 18S, 5S) each hybridized with several mtDNA fragments in a particular restriction digest (Eco RI, Xho I, or Sal I). In each case, the DNA fragments to which 18S and 5S rRNAs hybridized were the same, but different from those to which 26S rRNA hybridized. From these results, we conclude that the structural genes for wheat mitochondrial 18S and 5S rRNAs are closely linked, but are physically distant from the genes for wheat mitochondrial 26S rRNA. This arrangement of rRNA genes is clearly different from that in prokaryotes and chloroplasts, where 23S, 16S and 5S rRNA genes are closely linked, even though wheat mitochondrial 18S rRNA has previously been shown to be prokaryotic in nature. The mixed population of wheat mitochondrial 4S RNAs (tRNAs) hybridized with many large restriction fragments, indicating that the tRNA genes are broadly distributed throughout the mitochondrial genome, with some apparent clustering in regions containing 18S and 5S rRNA genes.
我们在此表明，小麦（普通小麦Vill. [小麦L.] 品种撒切尔）的线粒体特异性核糖体RNA和转运RNA由线粒体DNA（mtDNA）编码。单个小麦线粒体rRNA种类（26S、18S、5S）在特定的限制性消化（Eco RI、Xho I或Sal I）中各自与几个mtDNA片段杂交。在每种情况下，18S和5S rRNA杂交的DNA片段相同，但与26S rRNA杂交的片段不同。从这些结果我们得出结论，小麦线粒体18S和5S rRNA的结构基因紧密相连，但在物理位置上与小麦线粒体26S rRNA的基因相距较远。rRNA基因的这种排列明显不同于原核生物和叶绿体中的排列，在原核生物和叶绿体中23S、16S和5S rRNA基因紧密相连，尽管之前已表明小麦线粒体18S rRNA本质上是原核生物的。小麦线粒体4S RNA（tRNA）的混合群体与许多大的限制性片段杂交，表明tRNA基因广泛分布于整个线粒体基因组中，在含有18S和5S rRNA基因的区域有一些明显的聚集。
