Tong Q, Dalgin G, Xu H, Ting C N, Leiden J M, Hotamisligil G S
Division of Biological Sciences and Department of Nutrition, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA.
Science. 2000 Oct 6;290(5489):134-8. doi: 10.1126/science.290.5489.134.
Genes that control the early stages of adipogenesis remain largely unknown. Here, we show that murine GATA-2 and GATA-3 are specifically expressed in white adipocyte precursors and that their down-regulation sets the stage for terminal differentiation. Constitutive GATA-2 and GATA-3 expression suppressed adipocyte differentiation and trapped cells at the preadipocyte stage. This effect is mediated, at least in part, through the direct suppression of peroxisome proliferator-activated receptor gamma. GATA-3-deficient embryonic stem cells exhibit an enhanced capacity to differentiate into adipocytes, and defective GATA-2 and GATA-3 expression is associated with obesity. Thus, GATA-2 and GATA-3 regulate adipocyte differentiation through molecular control of the preadipocyte-adipocyte transition.
控制脂肪生成早期阶段的基因在很大程度上仍然未知。在此，我们表明小鼠GATA-2和GATA-3在白色脂肪细胞前体中特异性表达，并且它们的下调为终末分化奠定了基础。组成型GATA-2和GATA-3表达抑制脂肪细胞分化，并将细胞困在前脂肪细胞阶段。这种效应至少部分是通过直接抑制过氧化物酶体增殖物激活受体γ介导的。GATA-3缺陷的胚胎干细胞表现出增强的分化为脂肪细胞的能力，并且GATA-2和GATA-3表达缺陷与肥胖相关。因此，GATA-2和GATA-3通过对前脂肪细胞-脂肪细胞转变的分子控制来调节脂肪细胞分化。
