Wickström C, Christersson C, Davies J R, Carlstedt I
Mucosal Biology Group, Department of Cell and Molecular Biology, Section for Molecular Pathogenesis, Lund University, P.O. Box 94, S-22100 Lund, Sweden.
Biochem J. 2000 Oct 15;351 Pt 2(Pt 2):421-8.
Stimulated human submandibular/sublingual (HSMSL) and whole saliva were separated into sol and gel phases and mucins were isolated by density-gradient centrifugation in CsCl/4M guanidinium chloride. MUC5B and MUC7 were identified using anti-peptide antisera raised against sequences within the MUC5B and MUC7 apoproteins respectively. MUC7 was found mainly in the sol phase of both HSMSL and whole saliva, but some MUC7 was consistently present in the gel phase, suggesting that this mucin may interact with the salivary gel matrix. In HSMSL saliva, MUC5B was found in the gel phase; however, most of the material was 'insoluble' in guanidinium chloride and was only brought into solution by reduction. In whole saliva, the MUC5B mucin was present both in the sol and gel phases although some material was again 'insoluble'. Rate-zonal centrifugation of whole saliva showed that MUC5B mucins in the sol phase were smaller than those in the gel phase, suggesting differences in oligomerization and/or degradation. Antibodies against IgA, secretory component, lysozyme and lactoferrin were used to study the distribution of non-gel-forming proteins in the different phases of saliva. The majority of these proteins was found in the sol phase of both HSMSL and whole saliva. However, a significant fraction was present in the gel phase of whole saliva, suggesting a post-secretory interaction with the salivary gel matrix. A monoclonal antibody against a parotid salivary agglutinin was used to show that this protein is present mainly in the gel phase of both whole saliva and parotid secretion.
将刺激后的人类颌下腺/舌下腺（HSMSL）唾液和全唾液分离为溶胶相和凝胶相，通过在CsCl/4M氯化胍中进行密度梯度离心分离粘蛋白。分别使用针对MUC5B和MUC7载脂蛋白内序列产生的抗肽抗血清鉴定MUC5B和MUC7。发现MUC7主要存在于HSMSL唾液和全唾液的溶胶相中，但在凝胶相中始终存在一些MUC7，这表明该粘蛋白可能与唾液凝胶基质相互作用。在HSMSL唾液中，MUC5B存在于凝胶相中；然而，大部分物质在氯化胍中“不溶”，只有通过还原才能溶解。在全唾液中，MUC5B粘蛋白同时存在于溶胶相和凝胶相中，尽管有些物质再次“不溶”。全唾液的速率区带离心显示，溶胶相中的MUC5B粘蛋白比凝胶相中的小，这表明在寡聚化和/或降解方面存在差异。使用针对IgA、分泌成分、溶菌酶和乳铁蛋白的抗体来研究非凝胶形成蛋白在唾液不同相中的分布。这些蛋白中的大多数存在于HSMSL唾液和全唾液的溶胶相中。然而，全唾液凝胶相中存在相当一部分，这表明与唾液凝胶基质存在分泌后相互作用。使用针对腮腺唾液凝集素的单克隆抗体表明，该蛋白主要存在于全唾液和腮腺分泌物的凝胶相中。
