Selinger S L, Bland R D, Demling R H, Staub N C
J Appl Physiol. 1975 Nov;39(5):773-9. doi: 10.1152/jappl.1975.39.5.773.
We measured the steady-state volumes of distribution for radioactive chloride, sucrose, and albumin in the lung of six anesthetized, spen-thorax sheep. We allowed 2 days for [131I]albumin to equilibrate throughout the body, 2 h for the 36Cl, and a 40-min constant infusion for [14C]sucrose before killing the animal. Five minutes before death, we gave [125I]albumin to tag lung plasma volume. We killed the animals by clamping both lung hila; we then removed the lungs and homogenized them. We measured residual red cell and plasma volume, total extravascular lung water, and the extravascular content of the three tracers. The distribution volumes expressed as fractions of blood-free lung weight were: 36Cl equals 0.44, sucrose equals 0.28, and albumin equals 0.07. If the sucrose distribution volume is taken as the best estimate of the lung's extravascular extracellular space, then chloride clearly overestimates the interstitial fluid volume, being either bound or partially intracellular. On the other hand, albumin clearly underestimates the interstitial fluid volume.
我们测定了六只麻醉开胸绵羊肺中放射性氯化物、蔗糖和白蛋白的稳态分布容积。我们让[131I]白蛋白在全身达到平衡用了2天，36Cl用了2小时，在处死动物前，[14C]蔗糖持续输注40分钟。在 animal death 前5分钟，我们给予[125I]白蛋白标记肺血浆容积。我们通过钳夹双侧肺门处死动物；然后取出肺并将其匀浆。我们测量了残余红细胞和血浆容积、肺血管外总水量以及三种示踪剂的血管外含量。以无血肺重量的分数表示的分布容积为：36Cl等于0.44，蔗糖等于0.28，白蛋白等于0.07。如果将蔗糖分布容积作为肺血管外细胞外间隙的最佳估计值，那么氯化物显然高估了间质液容积，它要么被结合要么部分存在于细胞内。另一方面，白蛋白明显低估了间质液容积。 （注：原文中“spen-thorax”疑似拼写错误，推测为“open-thorax”，翻译时按推测内容进行了调整，若实际不是该词，请根据正确内容修改译文。）
