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质粒DNA与蛋白质的松弛复合体。I. 质粒ColE1和ColE2松弛复合体中蛋白质与DNA的链特异性结合

Relaxation complexes of plasmid DNA and protein. I. Strand-specific association of protein and DNA in the relaxed complexes of plasmids ColE1 and ColE2.

作者信息

Blair D G, Helinski D R

出版信息

J Biol Chem. 1975 Nov 25;250(22):8785-9.

PMID:1102543
Abstract

The ColE1 and ColE2 relaxation complexes of supercoiled DNA and protein were purified from Escherichia coli cells. Protein remains associated with the open circular DNA of these complexes after induction of relaxation with sodium dodecyl sulfate. The protein is associated specifically with the strand that possesses a site-specific break in the Co1E1 and ColE2 relaxed complexes. This protein remains associated with the DNA after centrifugation of the relaxed complex in a neutral or alkaline (pH 12.5) cesium chloride gradient or treatment with 8 M urea, 2 M NaSCN, 2M LiCl, 0.2 M sodium acetate, pH 4.6, and 70% formamide at 60 degrees.

摘要

从大肠杆菌细胞中纯化出超螺旋DNA与蛋白质的ColE1和ColE2松弛复合体。在用十二烷基硫酸钠诱导松弛后，蛋白质仍与这些复合体的开环DNA结合。该蛋白质与在ColE1和ColE2松弛复合体中具有位点特异性断裂的链特异性结合。在用中性或碱性（pH 12.5）氯化铯梯度对松弛复合体进行离心，或用8M尿素、2M硫氰酸钠、2M氯化锂、0.2M乙酸钠（pH 4.6）和60℃下的70%甲酰胺处理后，该蛋白质仍与DNA结合。

相似文献

1
Relaxation complexes of plasmid DNA and protein. I. Strand-specific association of protein and DNA in the relaxed complexes of plasmids ColE1 and ColE2.质粒DNA与蛋白质的松弛复合体。I. 质粒ColE1和ColE2松弛复合体中蛋白质与DNA的链特异性结合
J Biol Chem. 1975 Nov 25;250(22):8785-9.
2
Relaxation complexes of plasmid DNA and protein. II. Characterization of the proteins associated with the unrelaxed and relaxed complexes of plasmid ColE1.质粒DNA与蛋白质的松弛复合物。II. 与质粒ColE1的未松弛和松弛复合物相关的蛋白质的特性
J Biol Chem. 1975 Nov 25;250(22):8790-5.
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Relaxation complexes of plasmids ColE1 and ColE2: unique site of the nick in the open circular DNA of the relaxed complexes.质粒ColE1和ColE2的松弛复合体：松弛复合体开环DNA中切口的独特位点。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3854-7. doi: 10.1073/pnas.71.10.3854.
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Relaxation complexes of poasmid DNA and protein. III. Association of protein with the 5' terminus of the broken DNA strand in the relaxed complex of plasmid ColE1.质粒DNA与蛋白质的松弛复合体。III. 蛋白质与质粒ColE1松弛复合体中断裂DNA链5'末端的结合
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Strand-specific supercoiled DNA-protein relaxation complexes: comparison of the complexes of bacterial plasmids ColE1 and ColE2.链特异性超螺旋DNA-蛋白质松弛复合物：细菌质粒ColE1和ColE2复合物的比较。
Proc Natl Acad Sci U S A. 1971 Jan;68(1):210-4. doi: 10.1073/pnas.68.1.210.
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Properties of the relaxation complex of supercoiled deoxyribonucleic acid and protein of R plasmid NR1 in Escherichia coli.大肠杆菌中R质粒NR1的超螺旋脱氧核糖核酸与蛋白质松弛复合体的性质
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Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.环磷酸腺苷对质粒脱氧核糖核酸复制的刺激作用以及对质粒脱氧核糖核酸 - 蛋白质松弛复合物的分解代谢物阻遏作用。
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Studies of ColE1-plasmid DNA and its interactions with histones: sedimentation velocity studies of monodisperse complexes reconstituted with calf-thymus histones.大肠杆菌ColE1质粒DNA及其与组蛋白相互作用的研究：用小牛胸腺组蛋白重构的单分散复合物的沉降速度研究。
Nucleic Acids Res. 1977;4(5):1207-23. doi: 10.1093/nar/4.5.1207.

引用本文的文献

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Proc Natl Acad Sci U S A. 1981 Apr;78(4):2596-600. doi: 10.1073/pnas.78.4.2596.
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Location and nucleotide sequence of the transfer origin of the broad host range plasmid RK2.广宿主范围质粒RK2转移起始点的定位及核苷酸序列
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3595-8. doi: 10.1073/pnas.80.12.3595.
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Processing of plasmid DNA during bacterial conjugation.细菌接合过程中质粒DNA的加工
Microbiol Rev. 1984 Mar;48(1):24-41. doi: 10.1128/mr.48.1.24-41.1984.
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Association of the virD2 protein with the 5' end of T strands in Agrobacterium tumefaciens.根癌土壤杆菌中virD2蛋白与T链5'端的关联。
J Bacteriol. 1988 Aug;170(8):3367-74. doi: 10.1128/jb.170.8.3367-3374.1988.
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EMBO J. 1988 Dec 20;7(13):4055-62. doi: 10.1002/j.1460-2075.1988.tb03299.x.
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Mol Gen Genet. 1986 Jun;203(3):496-504. doi: 10.1007/BF00422076.
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