质粒ColE1和ColE2的松弛复合体：松弛复合体开环DNA中切口的独特位点。
Relaxation complexes of plasmids ColE1 and ColE2: unique site of the nick in the open circular DNA of the relaxed complexes.
作者信息
Lovett M A, Guiney D G, Helinski D R
出版信息
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3854-7. doi: 10.1073/pnas.71.10.3854.
Abstract
The product of the induced relaxation of supercoiled DNA-protein relaxation complexes of colicinogenic factors E1 (ColE1) and E2 (ColE2) is an open circular DNA molecule with a strand-specific nick. Cleavage of the open circular DNA of each relaxed complex with the EcoRI restriction endonuclease demonstrates that the single-strand break is at a unique position. The site of the single-strand break in the relaxed ColE1 complex is approximately the same distance from the EcoRI cleavage site as the origin of ColE1 DNA replication.
摘要
产大肠杆菌素因子E1（ColE1）和E2（ColE2）的超螺旋DNA-蛋白质松弛复合物诱导松弛的产物是一种具有链特异性切口的开环DNA分子。用EcoRI限制性内切酶切割每个松弛复合物的开环DNA表明，单链断裂位于一个独特的位置。松弛的ColE1复合物中单链断裂的位点与ColE1 DNA复制起点到EcoRI切割位点的距离大致相同。
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