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1
Relaxation complexes of plasmids ColE1 and ColE2: unique site of the nick in the open circular DNA of the relaxed complexes.质粒ColE1和ColE2的松弛复合体：松弛复合体开环DNA中切口的独特位点。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3854-7. doi: 10.1073/pnas.71.10.3854.
2
Strand-specific supercoiled DNA-protein relaxation complexes: comparison of the complexes of bacterial plasmids ColE1 and ColE2.链特异性超螺旋DNA-蛋白质松弛复合物：细菌质粒ColE1和ColE2复合物的比较。
Proc Natl Acad Sci U S A. 1971 Jan;68(1):210-4. doi: 10.1073/pnas.68.1.210.
3
Relaxation complexes of plasmid DNA and protein. I. Strand-specific association of protein and DNA in the relaxed complexes of plasmids ColE1 and ColE2.质粒DNA与蛋白质的松弛复合体。I. 质粒ColE1和ColE2松弛复合体中蛋白质与DNA的链特异性结合
J Biol Chem. 1975 Nov 25;250(22):8785-9.
4
Properties of the relaxation complexes of supercoiled deoxyribonucleic acid and protein of the R plasmids R64, R28K, and R6K.R质粒R64、R28K和R6K的超螺旋脱氧核糖核酸与蛋白质的松弛复合物的性质
J Bacteriol. 1974 Oct;120(1):545-8. doi: 10.1128/jb.120.1.545-548.1974.
5
Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.环磷酸腺苷对质粒脱氧核糖核酸复制的刺激作用以及对质粒脱氧核糖核酸 - 蛋白质松弛复合物的分解代谢物阻遏作用。
J Bacteriol. 1973 May;114(2):577-91. doi: 10.1128/jb.114.2.577-591.1973.
6
Location of non-DNA components of closed circular colicin E1 plasmid DNA.闭合环状大肠杆菌素E1质粒DNA非DNA组分的定位
Nature. 1975 Feb 20;253(5493):652-4. doi: 10.1038/253652a0.
7
Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication.大肠杆菌素E1质粒DNA在细胞提取物中的复制。复制起点及方向
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2260-4. doi: 10.1073/pnas.71.6.2260.
8
Mapping of colicin E2 and colicin E3 plasmid deoxyribonucleic acid EcoR-1-sensitive sites.大肠杆菌素E2和大肠杆菌素E3质粒脱氧核糖核酸EcoR-1敏感位点的图谱分析
J Bacteriol. 1975 Jan;121(1):381-9. doi: 10.1128/jb.121.1.381-389.1975.
9
Bidirectional replication of plasmid R6K DNA in Escherichia coli; correspondence between origin of replication and position of single-strand break in relaxed complex.质粒R6K DNA在大肠杆菌中的双向复制；复制起点与松弛复合物中单链断裂位置之间的对应关系。
Proc Natl Acad Sci U S A. 1975 Aug;72(8):2905-9. doi: 10.1073/pnas.72.8.2905.
10
Relaxation complexes of plasmid DNA and protein. II. Characterization of the proteins associated with the unrelaxed and relaxed complexes of plasmid ColE1.质粒DNA与蛋白质的松弛复合物。II. 与质粒ColE1的未松弛和松弛复合物相关的蛋白质的特性
J Biol Chem. 1975 Nov 25;250(22):8790-5.

引用本文的文献

1
Cloning the origin of transfer region of the resistance plasmid R1.克隆抗性质粒R1转移区域的起源
EMBO J. 1984 Aug;3(8):1731-5. doi: 10.1002/j.1460-2075.1984.tb02039.x.
2
Localization and nucleotide sequence of the bom region of Clo DF13.Clo DF13的bom区域的定位及核苷酸序列
Mol Gen Genet. 1983;192(3):444-51. doi: 10.1007/BF00392189.
3
Plasmid-encoded regulation of colicin E1 gene expression.质粒编码对大肠杆菌素E1基因表达的调控。
J Bacteriol. 1983 Nov;156(2):487-92. doi: 10.1128/jb.156.2.487-492.1983.
4
Molecular cloning of two fast myosin heavy chain cDNAs from chicken embryo skeletal muscle.从鸡胚骨骼肌中克隆出两个快速肌球蛋白重链cDNA
Proc Natl Acad Sci U S A. 1981 May;78(5):2843-7. doi: 10.1073/pnas.78.5.2843.
5
Location of two relaxation nick sites in R6K and single sites in pSC101 and RSF1010 close to origins of vegetative replication: implication for conjugal transfer of plasmid deoxyribonucleic acid.R6K中两个松弛切口位点以及pSC101和RSF1010中靠近营养复制起点的单个位点的位置：对质粒脱氧核糖核酸接合转移的影响。
J Bacteriol. 1980 Dec;144(3):923-32. doi: 10.1128/jb.144.3.923-932.1980.
6
Insertion element IS102 resides in plasmid pSC101.插入元件IS102存在于质粒pSC101中。
J Bacteriol. 1980 Oct;144(1):131-40. doi: 10.1128/jb.144.1.131-140.1980.
7
Human cytoplasmic superoxide dismutase cDNA clone: a probe for studying the molecular biology of Down syndrome.人细胞质超氧化物歧化酶cDNA克隆：用于研究唐氏综合征分子生物学的探针。
Proc Natl Acad Sci U S A. 1982 May;79(9):2808-11. doi: 10.1073/pnas.79.9.2808.
8
Processing of plasmid DNA during bacterial conjugation.细菌接合过程中质粒DNA的加工
Microbiol Rev. 1984 Mar;48(1):24-41. doi: 10.1128/mr.48.1.24-41.1984.
9
Molecular cloning of mRNA sequences for cardiac alpha- and beta-form myosin heavy chains: expression in ventricles of normal, hypothyroid, and thyrotoxic rabbits.心脏α和β型肌球蛋白重链mRNA序列的分子克隆：在正常、甲状腺功能减退和甲状腺功能亢进兔子心室中的表达
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5847-51. doi: 10.1073/pnas.79.19.5847.
10
Molecular characterization of the virD operon from Agrobacterium tumefaciens.根癌土壤杆菌virD操纵子的分子特征分析
Nucleic Acids Res. 1987 Sep 25;15(18):7503-17. doi: 10.1093/nar/15.18.7503.

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SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.DNA大小与形状的沉降研究
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A restriction enzyme from Hemophilus influenzae. II.一种来自流感嗜血杆菌的限制性内切酶。II.
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A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.一种用于检测和分离闭环双链DNA的染料浮力密度法：HeLa细胞中的闭环DNA
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Formation of the parental replicative form DNA of bacteriophage phi-X174 and initial events in its replication.噬菌体φ-X174亲本复制型DNA的形成及其复制的起始事件。
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The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis.噬菌体T5的染色体。I. 用琼脂糖凝胶电泳分析单链DNA片段
J Mol Biol. 1972 Feb 14;63(3):383-95. doi: 10.1016/0022-2836(72)90435-4.
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F 1 sex factor of Escherichia coli. Size and purification in the form of a strand-specific relaxation complex of supercoiled deoxyribonucleic acid and protein.大肠杆菌的F1性因子。以超螺旋脱氧核糖核酸与蛋白质的链特异性松弛复合体形式存在的大小及纯化方法。
Biochemistry. 1971 Dec 21;10(26):4975-80. doi: 10.1021/bi00802a022.
7
Strand-specific supercoiled DNA-protein relaxation complexes: comparison of the complexes of bacterial plasmids ColE1 and ColE2.链特异性超螺旋DNA-蛋白质松弛复合物：细菌质粒ColE1和ColE2复合物的比较。
Proc Natl Acad Sci U S A. 1971 Jan;68(1):210-4. doi: 10.1073/pnas.68.1.210.
8
Evidence for the existence of the colicinogenic factors E2 and E3 as supercoiled circular DNA-protein relaxation complexes.产大肠杆菌素因子E2和E3以超螺旋环状DNA-蛋白质松弛复合物形式存在的证据。
Biochem Biophys Res Commun. 1970 Aug 11;40(3):608-13. doi: 10.1016/0006-291x(70)90947-2.
9
The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation.通过聚丙烯酰胺凝胶电泳测定核糖核酸的分子量。构象变化的影响。
Biochem J. 1969 Jun;113(1):131-8. doi: 10.1042/bj1130131.
10
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.大肠杆菌中的超螺旋环状DNA-蛋白质复合物：纯化及诱导转化为开放环状DNA形式
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66. doi: 10.1073/pnas.62.4.1159.

质粒ColE1和ColE2的松弛复合体：松弛复合体开环DNA中切口的独特位点。

Relaxation complexes of plasmids ColE1 and ColE2: unique site of the nick in the open circular DNA of the relaxed complexes.

作者信息

Lovett M A, Guiney D G, Helinski D R

出版信息

Proc Natl Acad Sci U S A. 1974 Oct;71(10):3854-7. doi: 10.1073/pnas.71.10.3854.

DOI:10.1073/pnas.71.10.3854
PMID:4610563
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC434282/
Abstract

The product of the induced relaxation of supercoiled DNA-protein relaxation complexes of colicinogenic factors E1 (ColE1) and E2 (ColE2) is an open circular DNA molecule with a strand-specific nick. Cleavage of the open circular DNA of each relaxed complex with the EcoRI restriction endonuclease demonstrates that the single-strand break is at a unique position. The site of the single-strand break in the relaxed ColE1 complex is approximately the same distance from the EcoRI cleavage site as the origin of ColE1 DNA replication.

摘要

产大肠杆菌素因子E1（ColE1）和E2（ColE2）的超螺旋DNA-蛋白质松弛复合物诱导松弛的产物是一种具有链特异性切口的开环DNA分子。用EcoRI限制性内切酶切割每个松弛复合物的开环DNA表明，单链断裂位于一个独特的位置。松弛的ColE1复合物中单链断裂的位点与ColE1 DNA复制起点到EcoRI切割位点的距离大致相同。