大肠杆菌中R质粒NR1的超螺旋脱氧核糖核酸与蛋白质松弛复合体的性质

Properties of the relaxation complex of supercoiled deoxyribonucleic acid and protein of R plasmid NR1 in Escherichia coli.

作者信息

Womble D D, Rownd R H

出版信息

J Bacteriol. 1977 Jul;131(1):145-52. doi: 10.1128/jb.131.1.145-152.1977.

DOI:10.1128/jb.131.1.145-152.1977

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC235403/

Abstract

Some properties of the supercoiled deoxyribonucleic acid (DNA)-protein relaxation complex of the R plasmid NR1, which contains more than one origin for DNA replication, were examined. The percentage of complexed NR1 molecules that can be converted to the relaxed (nicked) form appeared to be unaffected by the conditions under which the host cells were cultured. However, the percentage of supercoiled NR1 DNA that can be relaxed was highly dependent on the method used to prepare the DNA and the agents used to induce relaxation. Our data suggest that 100% of NR1 molecules may exist in situ as DNA-protein relaxation complexes. An RTF-Tc segregant of NR1, which has deleted the r-determinants component of the NR1 and therefore does not contain the two origins of replication located in the r-determinants, has indistinguishable relaxation properties in comparison with NR1 itself.

摘要

对含有多个DNA复制起点的R质粒NR1的超螺旋脱氧核糖核酸（DNA）-蛋白质松弛复合体的一些特性进行了研究。可转化为松弛（带切口）形式的复合NR1分子的百分比似乎不受宿主细胞培养条件的影响。然而，能够被松弛的超螺旋NR1 DNA的百分比高度依赖于制备DNA所使用的方法以及用于诱导松弛的试剂。我们的数据表明，100%的NR1分子可能以DNA-蛋白质松弛复合体的形式原位存在。NR1的一个RTF-Tc分离株，它删除了NR1的r-决定簇成分，因此不包含位于r-决定簇中的两个复制起点，与NR1本身相比，具有难以区分的松弛特性。

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本文引用的文献

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Mol Gen Genet. 1975;136(4):273-89. doi: 10.1007/BF00341713.

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Replication of R-factors in Proteus mirabilis: replication under relaxed control.奇异变形杆菌中R因子的复制：松弛控制下的复制

J Mol Biol. 1970 Aug;51(3):473-89. doi: 10.1016/0022-2836(70)90002-1.

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Non-chromosomal antibiotic resistance in bacteria. 3. Isolation of the discrete transfer unit of the R-factor R1.细菌中的非染色体抗生素抗性。3. R 因子 R1 离散转移单位的分离。

Proc Natl Acad Sci U S A. 1970 Oct;67(2):510-6. doi: 10.1073/pnas.67.2.510.

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The molecular nature and replication of drug resistance factors of the Enterobacteriaceae.肠杆菌科细菌耐药因子的分子本质与复制

Ann N Y Acad Sci. 1971 Jun 11;182:188-206. doi: 10.1111/j.1749-6632.1971.tb30656.x.

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Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M.大肠杆菌噬菌体及大肠杆菌噬菌体DNA的分子量。IV. 来自噬菌体T4、T5和T7的DNA的分子量以及分子量测定的一般问题

J Mol Biol. 1970 Dec 28;54(3):567-77. doi: 10.1016/0022-2836(70)90127-0.

6

Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.超螺旋脱氧核糖核酸-蛋白质松弛复合体的性质及松弛事件的链特异性

Biochemistry. 1970 Oct 27;9(22):4428-40. doi: 10.1021/bi00824a026.

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Biochem Biophys Res Commun. 1970 Oct 9;41(1):150-6. doi: 10.1016/0006-291x(70)90481-x.

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Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.大肠杆菌中的超螺旋环状DNA-蛋白质复合物：纯化及诱导转化为开放环状DNA形式

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