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用间接免疫铁蛋白技术在电子显微镜下鉴定沙眼衣原体的原体。

Identification of the elementary bodies of Chlamydia trachomatis in the electron microscope by an indirect immunoferritin technique.

作者信息

Ashley C R, Richmond S J, Caul E O

出版信息

J Clin Microbiol. 1975 Oct;2(4):327-31. doi: 10.1128/jcm.2.4.327-331.1975.

DOI:10.1128/jcm.2.4.327-331.1975
PMID:1102559
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC362804/
Abstract

An indirect immunoferritin (IIF) technique is described for recognizing the elementary bodies (EB) of Chlamydia trachomatis in unsectioned preparations. Both the EB of a genital strain of C. trachomatis grown in irradiated McCoy cells and EB in clinical specimens obtained from patients attending a venereal disease clinic were identified by the IIF test in the electron microscope. Cell culture-grown EB were detected by ferritin staining for up to 4 weeks after the organisms had lost their infectivity for tissue cultures. The IIF test was of comparable sensitivity to isolation methods in detecting chlamydiae in clinical specimens. Other possible applications of the IIF technique are discussed.

摘要

本文描述了一种间接免疫铁蛋白（IIF）技术，用于在未切片的制剂中识别沙眼衣原体的原体（EB）。通过电子显微镜下的IIF试验，鉴定了在经辐照的McCoy细胞中生长的沙眼衣原体生殖道菌株的EB以及从性病门诊患者获得的临床标本中的EB。在细胞培养物中生长的EB在对组织培养物失去感染性后，通过铁蛋白染色可检测长达4周。在检测临床标本中的衣原体时，IIF试验与分离方法具有相当的敏感性。还讨论了IIF技术的其他可能应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/362804/874075255ab9/jcm00239-0059-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/362804/66a92dae94f8/jcm00239-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/362804/9b82de509624/jcm00239-0059-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/362804/874075255ab9/jcm00239-0059-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/362804/66a92dae94f8/jcm00239-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/362804/9b82de509624/jcm00239-0059-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/362804/874075255ab9/jcm00239-0059-c.jpg

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引用本文的文献

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The rôle of Chlamydia trachomatis in genital-tract and associated diseases.沙眼衣原体在生殖道及相关疾病中的作用。
J Clin Pathol. 1980 Mar;33(3):205-33. doi: 10.1136/jcp.33.3.205.

本文引用的文献

1
FERRITIN-ANTIBODY CONJUGATES AS STAINS FOR ELECTRON MICROSCOPY.作为电子显微镜染色剂的铁蛋白-抗体缀合物
Lab Invest. 1963 Oct;12:1002-8.
2
A two-layer technique for detecting surface antigens in the sea urchin egg with ferritin-conjugated antibody.一种用铁蛋白偶联抗体检测海胆卵表面抗原的双层技术。
J Cell Biol. 1962 Jul;14(1):144-51. doi: 10.1083/jcb.14.1.144.
3
A negative staining method for high resolution electron microscopy of viruses.一种用于病毒高分辨率电子显微镜观察的负染色方法。
Biochim Biophys Acta. 1959 Jul;34:103-10. doi: 10.1016/0006-3002(59)90237-9.
4
Preparation of an electron-dense antibody conjugate.电子致密抗体偶联物的制备。
Nature. 1959 May 30;183(4674):1523-4. doi: 10.1038/1831523a0.
5
The relation of the psittacosis group (Chlamydiae) to bacteria and viruses.鹦鹉热菌群（衣原体）与细菌和病毒的关系。
Annu Rev Microbiol. 1966;20:107-30. doi: 10.1146/annurev.mi.20.100166.000543.
6
Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test.新型微量滴定间接免疫荧光试验中生殖系统沙眼衣原体、性病性淋巴肉芽肿及相关病原体之间的免疫学关系
Am J Ophthalmol. 1970 Sep;70(3):367-74. doi: 10.1016/0002-9394(70)90096-6.
7
Comparison of 22 nm virus from human faeces with animal parvoviruses.来自人类粪便的22纳米病毒与动物细小病毒的比较。
J Gen Virol. 1974 Mar;22(3):447-50. doi: 10.1099/0022-1317-22-3-447.
8
Growth of Chlamydia trachomatis in McCoy cells treated with cytochalasin B.沙眼衣原体在经细胞松弛素B处理的 McCoy 细胞中的生长。
Appl Microbiol. 1974 Dec;28(6):912-4. doi: 10.1128/am.28.6.912-914.1974.
9
The role of immunoglobulin in the neutralization of trachoma infectivity.免疫球蛋白在沙眼传染性中和中的作用。
J Immunol. 1974 Nov;113(5):1607-17.
10
The isolation of Chlamydia subgroup A (Chlamydia trachomatis) in irradiated McCoy cells.在经辐照的 McCoy 细胞中分离沙眼衣原体 A 亚群（沙眼衣原体）
Med Lab Technol. 1974 Jan;31(1):7-9.