沙眼衣原体在经细胞松弛素B处理的 McCoy 细胞中的生长。
Growth of Chlamydia trachomatis in McCoy cells treated with cytochalasin B.
作者信息
Sompolinsky D, Richmond S
出版信息
Appl Microbiol. 1974 Dec;28(6):912-4. doi: 10.1128/am.28.6.912-914.1974.
Abstract
When the fungal metabolite cytochalasin B was added to McCoy cells, multinucleated giant cells developed. Monolayers of these cells proved as efficient as irradiated cells for the growth of three different serotypes of Chlamydia trachomatis and for the primary isolation of chlamydiae from clinical specimens obtained from patients attending a venereal disease clinic. Cytochalasin treatment of McCoy cells provides a convenient alternative to irradiation and should be of value in the isolation of chlamydiae from the eye and genital tract.
摘要
当将真菌代谢产物细胞松弛素B添加到 McCoy 细胞中时，多核巨细胞形成。这些细胞单层在支持三种不同血清型沙眼衣原体生长以及从性病门诊患者的临床标本中进行衣原体的初次分离方面，证明与经辐射处理的细胞一样有效。用细胞松弛素处理 McCoy 细胞为辐射处理提供了一种便捷的替代方法，在从眼和生殖道分离衣原体方面应具有价值。
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本文引用的文献
1
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Lancet. 1959 May 2;1(7079):902-5. doi: 10.1016/s0140-6736(59)91307-8.
2
Studies on the etiology of trachoma with special reference to isolation of the virus in chick embryo.沙眼病因学研究，特别涉及鸡胚中病毒的分离
Chin Med J. 1957 Jun;75(6):429-47.
3
Fine structure of cytochalasin-induced multinucleated cells.
J Ultrastruct Res. 1971 Jul;36(1):191-204. doi: 10.1016/s0022-5320(71)80097-7.
4
Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test.新型微量滴定间接免疫荧光试验中生殖系统沙眼衣原体、性病性淋巴肉芽肿及相关病原体之间的免疫学关系
Am J Ophthalmol. 1970 Sep;70(3):367-74. doi: 10.1016/0002-9394(70)90096-6.
5
Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran.在用二乙氨基乙基葡聚糖处理的HeLa 229细胞中对沙眼衣原体相关微生物进行初次分离。
J Infect Dis. 1972 Jun;125(6):665-8. doi: 10.1093/infdis/125.6.665.
6
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9
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Med Lab Technol. 1974 Jan;31(1):7-9.
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