Chiron H, Drouet A, Lieutier F, Payer H D, Ernst D, Sandermann H
University of Orléans, Biology Department BP6749, F-45067 Orléans cedex 2, France.
Plant Physiol. 2000 Oct;124(2):865-72. doi: 10.1104/pp.124.2.865.
The S-adenosyl-L-methionine:pinosylvin-O-methyltransferase (PMT) gene was sequenced from Scots pine (Pinus sylvestris). The open reading frame is arranged in two exons spaced by one 102-bp intron. Promoter regulatory elements such as two "CAAT" boxes and one "TATA" box were identified. Several cis-regulatory elements were recognized: stress-responsive elements (Myb-responsive elements) as well as G, H, and GC boxes. Moreover, elicitor-responsive elements (W boxes) and a sequence resembling the simian virus 40 enhancer core were found. In phloem and needles of control trees, the transcripts of stilbene synthase (STS) and PMT were hardly detectable. Increased ozone fumigation up to 0.3 microL L(-1) enhanced the transcript level of STS and PMT in needles but not in healthy phloem. Wounding, e.g. mock inoculation, of stem-phloem was characterized by a transient increase in STS and PMT transcripts, which was more pronounced in the case of fungal inoculation. Combination of fungal-challenge or mock treatment with ozone resulted in a positive interaction at 0.3 microL L(-1). Scots pine stilbene formation appeared to be induced via STS and PMT gene expression upon ozone and fungal stress as well as wounding. The broad stress-responsiveness is in agreement with the range of various cis-acting elements detected in the STS and PMT promoters.
从欧洲赤松（Pinus sylvestris）中对S-腺苷-L-甲硫氨酸：松芪醇-O-甲基转移酶（PMT）基因进行了测序。开放阅读框由两个外显子组成，中间间隔一个102bp的内含子。鉴定出了启动子调控元件，如两个“CAAT”框和一个“TATA”框。识别出了几个顺式调控元件：应激反应元件（Myb反应元件）以及G、H和GC框。此外，还发现了诱导子反应元件（W框）和一段类似于猿猴病毒40增强子核心的序列。在对照树的韧皮部和针叶中，几乎检测不到芪合酶（STS）和PMT的转录本。臭氧熏蒸浓度增加至0.3 μL L⁻¹可提高针叶中STS和PMT的转录水平，但对健康韧皮部则无此作用。对茎韧皮部进行创伤处理，如模拟接种，其特征是STS和PMT转录本短暂增加，在真菌接种的情况下更为明显。真菌激发或模拟处理与臭氧联合作用在0.3 μL L⁻¹时产生了正向相互作用。欧洲赤松芪的形成似乎是在臭氧、真菌胁迫以及创伤作用下通过STS和PMT基因表达诱导产生的。广泛的应激反应性与在STS和PMT启动子中检测到的各种顺式作用元件的范围一致。
