Hiraga S, Saito T
Mol Gen Genet. 1975;137(3):239-48. doi: 10.1007/BF00333019.
When E. coli F+ cells carrying the dna-167 or dnaC2 mutation, which causes the temperature-sensitive initiation of DNA replication, are exposed to a non-permissive temperature to stop the replication of chromosome and F factor, and then transferred back to a permissive temperature with the addition of chloramphenicol, one round of the chromosomal replication occurs, but further replication is inhibited. Under these conditions, F DNA replicates coincidentally with the initiation of the chromosomal replication in both strains. When rifampicin is added to the cells upon lowering of the temperature, the chromosome can not replicate in the F+ dna-167 strain, but can do so in the F+ dnaC2 strain. F DNA can replicate in both of the mutant strains under these conditions.
携带dna - 167或dnaC2突变（导致DNA复制温度敏感起始）的大肠杆菌F + 细胞，在暴露于非允许温度以停止染色体和F因子复制后，再转移到允许温度并添加氯霉素，会发生一轮染色体复制，但进一步的复制受到抑制。在这些条件下，两种菌株中F DNA都与染色体复制起始同时进行复制。当温度降低时向细胞中添加利福平，F + dna - 167菌株中的染色体无法复制，但F + dnaC2菌株中的染色体可以复制。在这些条件下，F DNA在两种突变菌株中都能复制。
