对大肠杆菌dnaC突变体中F质粒复制的重新检测。
Re-examination of F plasmid replication in a dnaC mutant of Escherichia coli.
作者信息
Van Brunt J, Waggoner B T, Pato M L
出版信息
Mol Gen Genet. 1977 Feb 15;150(3):285-92. doi: 10.1007/BF00268127.
PMID:321937
Abstract
The replication of an F' plasmid in a dnaC mutant, thermolabile for initiation of chromosomal replication, has been re-examined using a novel DNA-DNA annealing assay. Plasmid replication ceases rapidly at non-permissive conditions, consistent with a direct role for the dnaC product in the replication of F.
摘要
利用一种新型的DNA-DNA退火分析法，对F'质粒在dnaC突变体中的复制情况进行了重新研究，该突变体对染色体复制的起始具有温度敏感性。在非允许条件下，质粒复制迅速停止，这与dnaC产物在F质粒复制中起直接作用相一致。
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