能够在大肠杆菌高频重组（Hfr）菌株中复制的新型F'因子。
Novel F prime factors able to replicate in Escherichia coli Hfr strains.
作者信息
Hiraga S
出版信息
Proc Natl Acad Sci U S A. 1976 Jan;73(1):198-202. doi: 10.1073/pnas.73.1.198.
Abstract
A novel type of F' plasmid that can replicate extrachromosomally in cells of Hfr strains has been isolated. Genetic analysis of these plasmids indicates that all carry the dnaA-bglA region of the E. coli chromosome as well as ilv+ used as a selective marker. It is suggested that a specific site, designated poh+ (permissive on Hfr), is located in this region, and is essential for these plasmids to replicate in Hfr cells. The most interesting explanation, among others, would be that the poh+ site represents the replication origin of the bacterial chromosome.
摘要
一种新型的F'质粒已被分离出来，它能够在高频重组（Hfr）菌株的细胞中进行染色体外复制。对这些质粒的遗传分析表明，它们都携带大肠杆菌染色体的dnaA - bglA区域以及用作选择标记的ilv+。有人提出，在这个区域存在一个特定的位点，命名为poh+（在Hfr上可许可），它对于这些质粒在Hfr细胞中复制至关重要。其中最有趣的一种解释是，poh+位点代表了细菌染色体的复制起点。
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