Elseviers D, Gorini L
Mol Gen Genet. 1975;137(4):277-87. doi: 10.1007/BF00703254.
We describe a method for the direct selection of E. coli mutants restricting efficiency of suppression and misreading levels using a T4-coded nonsense suppressor. One mutant isolated has the phenotype expected for a restrictive mutant and may be ribosomal. Other possibilities are discussed.
我们描述了一种使用T4编码的无义抑制基因直接筛选限制抑制效率和错读水平的大肠杆菌突变体的方法。分离出的一个突变体具有限制突变体预期的表型,可能是核糖体相关的。还讨论了其他可能性。