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从纤细裸藻叶绿体中克隆的核糖体RNA基因。

Cloned ribosomal RNA genes from chloroplasts of Euglena gracilis.

作者信息

Lomax M I, Helling R B, Hecker L I, Schwartzbach S D, Barnett W E

出版信息

Science. 1977 Apr 8;196(4286):202-5. doi: 10.1126/science.403604.

Abstract

Fragments of Euglena chloroplast DNA generated by endonuclease R-Eco RI were separated by agarose-gel electrophoresis into 24 distinct bands. At least five fragments contain sequences complementary to chloroplast ribosomal RNA, Most of the Eco RI fragments have been cloned in a plasmid of Escherichia coli. Three of the cloned fragments were shown to contain chloroplast ribosomal RNA sequences by DNA-RNA hybridization.

摘要

由核酸内切酶R-Eco RI产生的眼虫叶绿体DNA片段,通过琼脂糖凝胶电泳被分离成24条不同的条带。至少有五个片段含有与叶绿体核糖体RNA互补的序列。大多数Eco RI片段已被克隆到大肠杆菌的质粒中。通过DNA-RNA杂交显示,其中三个克隆片段含有叶绿体核糖体RNA序列。

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