McKessar S J, Berry A M, Bell J M, Turnidge J D, Paton J C
Molecular Microbiology Unit, Women's and Children's Hospital, North Adelaide, South Australia 5006, Australia.
Antimicrob Agents Chemother. 2000 Nov;44(11):3224-8. doi: 10.1128/AAC.44.11.3224-3228.2000.
Enterococcus faecalis strain WCH9 displays a moderate level of resistance to vancomycin (MIC = 16 microgram/ml) and full susceptibility to teicoplanin but is negative by PCR analysis using primers specific for all known enterococcal vancomycin resistance genotypes (vanA, vanB, vanC, vanD, and vanE). We have isolated and sequenced a novel putative vancomycin resistance locus (designated vanG), which contains seven open reading frames, from this strain. These are organized differently from those of all the other enterococcal van loci, and, furthermore, the individual vanG gene products exhibit less than 50% amino acid sequence identity to other van gene products.
粪肠球菌菌株WCH9对万古霉素表现出中度耐药性（最低抑菌浓度=16微克/毫升），对替考拉宁完全敏感，但使用针对所有已知肠球菌万古霉素耐药基因型（vanA、vanB、vanC、vanD和vanE）的引物进行PCR分析时呈阴性。我们从该菌株中分离并测序了一个新的假定万古霉素耐药基因座（命名为vanG），它包含七个开放阅读框。这些开放阅读框的组织方式与所有其他肠球菌van基因座不同，此外，单个vanG基因产物与其他van基因产物的氨基酸序列同一性低于50%。
