大鼠神经元制剂中的钠钙交换异构体：出生后发育过程中其表达的不同变化。

Na(+)-Ca(2+) exchanger isoforms in rat neuronal preparations: different changes in their expression during postnatal development.

作者信息

Sakaue M, Nakamura H, Kaneko I, Kawasaki Y, Arakawa N, Hashimoto H, Koyama Y, Baba A, Matsuda T

机构信息

Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, 565-0871, Osaka, Japan.

出版信息

Brain Res. 2000 Oct 27;881(2):212-6. doi: 10.1016/s0006-8993(00)02808-0.

DOI:10.1016/s0006-8993(00)02808-0

PMID:11036162

Abstract

We examined the relative amounts of Na(+)-Ca(2+) exchanger (NCX) isoform mRNAs in cultured neurons, astrocytes and developmental rat brain. NCX1 transcript was predominant in neurons and astrocytes, but NCX2 transcript was about four-fold higher than NCX1 or NCX3 transcript in adult rat cortex. NCX2 transcript in the cortex increased markedly during postnatal development, whereas NCX1 and NCX3 transcripts decreased. Na(+)-dependent 45Ca(2+) uptake in the cortical homogenate increased significantly during postnatal development.

摘要

我们检测了培养的神经元、星形胶质细胞以及发育中的大鼠脑内钠钙交换体（NCX）同工型mRNA的相对含量。NCX1转录本在神经元和星形胶质细胞中占主导，但在成年大鼠皮质中，NCX2转录本比NCX1或NCX3转录本高约四倍。皮质中的NCX2转录本在出生后发育过程中显著增加，而NCX1和NCX3转录本则减少。出生后发育期间，皮质匀浆中钠依赖性45Ca2+摄取显著增加。