Fliermans C B, Schmidt E L
Appl Microbiol. 1975 Oct;30(4):676-84. doi: 10.1128/am.30.4.676-684.1975.
Specific detection of a particular bacterium by immunofluorescence was combined with estimation of its metabolic activity by autoradiography. The nitrifying bacteria Nitrobacter agilis and N. winogradskyi were used as a model system. Nitrobacter were incubated with NaH14CO3 and 14CO2 prior to study. The same preparations made for autoradiograms were stained with fluorescent antibodies specific for the Nitrobacter species. Examination by epifluorescence and transmitted dark-field microscopy revealed Nitrobacter cells with and without associated silver grains. Direct detection and simultaneous evaluation of metabolic activity of Nitrobacter was demonstrated in pure cultures, in a simple mixed culture, and in a natural soil.
通过免疫荧光对特定细菌进行特异性检测，并结合放射自显影法对其代谢活性进行评估。以硝化细菌敏捷硝化杆菌和维氏硝化杆菌作为模型系统。在研究前，将硝化杆菌与NaH¹⁴CO₃和¹⁴CO₂一起培养。用于放射自显影片的相同制剂用针对硝化杆菌属的荧光抗体染色。通过落射荧光和透射暗视野显微镜检查发现，有和没有相关银颗粒的硝化杆菌细胞。在纯培养物、简单混合培养物和天然土壤中都证明了对硝化杆菌代谢活性的直接检测和同步评估。
